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Products:Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474)
Overview
Product name
Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker
See all Oligodendrocyte Specific Protein products (5) ...
Description
Rabbit polyclonal to Oligodendrocyte Specific Protein - Oligodendrocyte Marker
Specificity
Reacts specifically with 22 kDa protein derived from CNS samples.
Tested applications
IHC-P, WB, IHC-FoFrmore details
Cross reactivity
Reacts with
Mouse, Rat
Predicted to work with
Human
Immunogen
A 15 residue synthetic C-terminal peptide.
Positive control
WB: rat CNS and mouse brain and testis
General notes
The concentration depends on the batch, please enquire for current stock concentration.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole Serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Immunology >> Immune System Diseases >> Autoimmune
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/500.
WB: 1/200 - 1/5000.Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).(Block in Tris-buffered saline (TBS) containing 1% BSA overnight at 4C. Possible oligomerisation or polimerisation of the protein have been reported and can lead to high MW bands.)
IHC-FoFr
IHC-FoFr: 1/100IHC-FoFr: 1/100
Target
Relevance
Oligodendrocyte specific protein (OSP), also known Claudin 11, is the third most abundant component of central nervous system myelin. The claudin superfamily consists of many structurally related proteins. These proteins, which include claudin 1 through 18, are located in both epithelial and endothelial cells in all tight junction bearing tissues. Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. There is growing evidence that OSP/Claudin 11 determines the permeability between layers of myelin sheaths via focal adhesion. Its expression is highly regulated during development, suggesting that it may play an important role in the growth and differentiation of oligodendrocytes and other cells outside the CNS. In addition, the protein is a candidate autoantigen in the development of autoimmune demyelinating disease.
Cellular localization
Integral cell membrane protein. Tight junctions.
Alternative names
- Claudin 11 antibody
- Claudin 11 antibody
- claudin11 antibody
see all
Database links
- Entrez Gene: 5010 Human
- Entrez Gene: 18417 Mouse
- Entrez Gene: 84588 Rat
- Omim: 601326 Human
- SwissProt: O75508 Human
- SwissProt: Q60771 Mouse
- SwissProt: Q99P82 Rat
- Unigene: 31595 Human
see all
Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker images:
Immunohistochemistry - Free Floating - Oligodendrocyte Specific Protein OSP antibody - Oligodendrocyte Marker (ab7474)
Immunofluorescent staining for OSP obtained with OSP antibody (ab7474) in rat brain cortex. The picture shows oligodendrocyte immunostaining in the cingular cortex. Picture taken with X20 objective, cells stained are ~30microns in diameter. Animals were intracardially perfused with 4% PFA. Tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. Protocol : free floating IHC on 30um cryostat sections. Primary antibody ab7474 was used at 1/100 and incubated overnight at RT. Secondary antibody, Alexa fluor 488 at 1/1000, incubated for 2h at RT.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker(ab7474)
IHC image of ab7474 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7474, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474)
This product has been referenced in:
- McCabe MJet al. Gonadotropins regulate rat testicular tight junctions in vivo. Endocrinology 151:2911-22 (2010). WB; Rat.Read more (PubMed: 20357222) »
- Simard JMet al. Brief suppression of Abcc8 prevents autodestruction of spinal cord after trauma. Sci Transl Med 2:28ra29 (2010).Read more (PubMed: 20410530) »
See all 4 publications for this product
Publishing research using ab7474? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"