Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474)

Overview

  • Product nameAnti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker
    See all Oligodendrocyte Specific Protein primary antibodies
  • Description
    Rabbit polyclonal to Oligodendrocyte Specific Protein - Oligodendrocyte Marker
  • SpecificityReacts specifically with 22 kDa protein derived from CNS samples.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    A 15 residue synthetic C-terminal peptide.

  • Positive control
    • WB: rat CNS and mouse brain and testis
  • General notesThe concentration depends on the batch, please enquire for current stock concentration.

Properties

Applications

Our Abpromise guarantee covers the use of ab7474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
IHC-Fr Use at an assay dependent concentration.
WB 1/200 - 1/5000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). Block in Tris-buffered saline (TBS) containing 1% BSA overnight at 4C. Possible oligomerisation or polimerisation of the protein have been reported and can lead to high MW bands.
IHC-FoFr 1/100.

Target

Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker images

  • Immunofluorescent staining for OSP obtained with OSP antibody (ab7474) in rat brain cortex. The picture shows oligodendrocyte immunostaining in the cingular cortex. Picture taken with X20 objective, cells stained are ~30microns in diameter. Animals were intracardially perfused with 4% PFA. Tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. Protocol : free floating IHC on 30um cryostat sections. Primary antibody ab7474 was used at 1/100 and incubated overnight at RT. Secondary antibody, Alexa fluor 488 at 1/1000, incubated for 2h at RT.
  • IHC image of ab7474 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7474, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemical analysis of adult mouse testis tissue, staining Oligodendrocyte Specific Protein (red) with ab7474.

    Tissue was blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA) for 1 hour at 25°C. A TRITC-conjugated donkey anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.

    See Abreview

References for Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474)

This product has been referenced in:

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M citric acide/sodium citrate
Sample Sheep Tissue sections (1-2 month old testis)
Specification 1-2 month old testis
Permeabilization No
Fixative 4%PFA
Username

Abcam user community

Verified customer

Submitted Jan 19 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Sample Human Tissue sections (spinalcord)
Specification spinalcord
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 27 2014

Thank you for contacting us.
Oligodendrocyte-specific protein (OSP) is a putative four-transmembrane protein of 22kD. It is primarily expressed in oligodendrocytes of the CNS and Sertoli cells of testes in the adult mouse. OSP is the third most ab...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Tissue sections (adult testes)
Specification adult testes
Permeabilization No
Fixative 4 %
Username

Dr. Qing Wen

Verified customer

Submitted Dec 24 2012

Thank you for your message and for providing this further information and the images.

Reviewing the details, I appreciate the time you have spent on these experiments and would be pleased to arrange afree of charge replacementor credit note ...

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Thank you for your reply.

I look forward to receiving the images, I appreciate your cooperation.

I would appreciate if you are also able to provide further information regarding the secondary antibodies, as previously requested. T...

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Thank you for your reply and for kindly providing this further information.

Reviewing the details, I fully understand your concerns and itregrettable the results fromthese three antibodies have not been satisfactory. It is unusual for 3 anti...

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Thank you for your patience. My colleague is still out of office this week.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been repor...

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Thank you for your email.

Fixing in paraformaldehyde for more than 10-15 min will cross link the proteins to the point where antigen retrieval may be required to ensure the antibody has free access to bind and detect the protein. There is also...

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Thank you for your email and I am sorry to hear about the problem you are experiencing.

The protocol seems to fine to me, which is pretty much straight forward so the problem might be with the negative control cells you have chosen; these migh...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"