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Immunohistochemistry - Free Floating - Oligodendrocyte Specific Protein OSP antibody - Oligodendrocyte Marker (ab7474)
(enlarge)
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Product Name
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Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker
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See all Oligodendrocyte Specific Protein antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Oligodendrocyte Specific Protein - Oligodendrocyte Marker
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Immunogen
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A 15 residue synthetic C-terminal peptide.
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Reacts with
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Ms, Rat
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Predicted to react
(species key)
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Hu
(Due to sequence homology)
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Specificity
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Reacts specifically with 22 kDa protein derived from CNS samples.
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Tested applications
(see key)
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IHC-FoFr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/50 - 1/500. Use Bouin fixed tissue. Do not perform antigen retrieval. (see Hellani et al).
IHC-FoFr: 1/100. Following perfusion fixation fix tissue overnight in 4%PFA.
WB: 1/200 - 1/5000. Block in Tris-buffered saline (TBS) containing 1% BSA overnight at 4C. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). Possible oligomerisation or polimerisation of the protein have been reported and can lead to high MW bands.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: rat CNS and mouse brain and testis
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Cellular localization
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Integral cell membrane protein. Tight junctions.
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Research areas
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Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Immunology >> Immune System Diseases >> Autoimmune
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
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Relevance
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Oligodendrocyte specific protein (OSP), also known Claudin 11, is the third most abundant component of central nervous system myelin. The claudin superfamily consists of many structurally related proteins. These proteins, which include claudin 1 through 18, are located in both epithelial and endothelial cells in all tight junction bearing tissues. Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. There is growing evidence that OSP/Claudin 11 determines the permeability between layers of myelin sheaths via focal adhesion. Its expression is highly regulated during development, suggesting that it may play an important role in the growth and differentiation of oligodendrocytes and other cells outside the CNS. In addition, the protein is a candidate autoantigen in the development of autoimmune demyelinating disease.
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Alternative names Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None
Constituents: Whole Serum
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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The concentration depends on the batch, please enquire for current stock concentration.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker images, references, products related to ab7474 and other tools.
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Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker images:
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 Immunohistochemistry - Free Floating - Oligodendrocyte Specific Protein OSP antibody - Oligodendrocyte Marker (ab7474)
Immunofluorescent staining for OSP obtained with OSP antibody (ab7474) in rat brain cortex. The picture shows oligodendrocyte immunostaining in the cingular cortex. Picture taken with X20 objective, cells stained are ~30microns in diameter. Animals were intracardially perfused with 4% PFA. Tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. Protocol : free floating IHC on 30um cryostat sections. Primary antibody ab7474 was used at 1/100 and incubated overnight at RT. Secondary antibody, Alexa fluor 488 at 1/1000, incubated for 2h at RT.
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References for Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474)
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This product has been used in: (two most recent references)
Jagessar SA et al. Autoimmunity Against Myelin Oligodendrocyte Glycoprotein Is Dispensable for the Initiation Although Essential for the Progression of Chronic Encephalomyelitis in Common Marmosets. J Neuropathol Exp Neurol 67:326-340 (2008). WB; Mouse. PubMed: 18379435
Hellani A et al. Developmental and hormonal regulation of the expression of oligodendrocyte-specific protein/claudin 11 in mouse testis. Endocrinology 141:3012-9 (2000). PubMed: 10919290
If you publish research using ab7474 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Oligodendrocyte Specific Protein
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Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker - more information
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