You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474)

CodeSizePriceAbpointsAvailability
    
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker
See all Oligodendrocyte Specific Protein products (5) ...

Description

Rabbit polyclonal to Oligodendrocyte Specific Protein - Oligodendrocyte Marker

Specificity

Reacts specifically with 22 kDa protein derived from CNS samples.

Tested applications

IHC-P, WB, IHC-FoFrmore details

Cross reactivity

Reacts with

Mouse, Rat

Predicted to work with

Human

Immunogen

A 15 residue synthetic C-terminal peptide.

Positive control

WB: rat CNS and mouse brain and testis

General notes

The concentration depends on the batch, please enquire for current stock concentration.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: Whole Serum

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

  • Immunohistochemistry - Free Floating - Oligodendrocyte Specific Protein OSP antibody - Oligodendrocyte Marker (ab7474)Immunohistochemistry - Free Floating - Oligodendrocyte Specific Protein OSP antibody - Oligodendrocyte Marker (ab7474) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker(ab7474)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker(ab7474) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab7474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/500.

    IHC-P: 1/50 - 1/500.

  • ShowHide

    WB

     WB: 1/200 - 1/5000.Detec...Read more →

    WB: 1/200 - 1/5000.Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).(Block in Tris-buffered saline (TBS) containing 1% BSA overnight at 4C. Possible oligomerisation or polimerisation of the protein have been reported and can lead to high MW bands.)

  • IHC-FoFr

     IHC-FoFr: 1/100

    IHC-FoFr: 1/100

Target

Relevance

Oligodendrocyte specific protein (OSP), also known Claudin 11, is the third most abundant component of central nervous system myelin. The claudin superfamily consists of many structurally related proteins. These proteins, which include claudin 1 through 18, are located in both epithelial and endothelial cells in all tight junction bearing tissues. Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. There is growing evidence that OSP/Claudin 11 determines the permeability between layers of myelin sheaths via focal adhesion. Its expression is highly regulated during development, suggesting that it may play an important role in the growth and differentiation of oligodendrocytes and other cells outside the CNS. In addition, the protein is a candidate autoantigen in the development of autoimmune demyelinating disease.

Cellular localization

Integral cell membrane protein. Tight junctions.

Alternative names

  • Claudin 11 antibody
  • Claudin 11 antibody
  • claudin11 antibody
  • CLDN 11 antibody
  • CLDN11 antibody
  • Oligodendrocyte transmembrane protein antibody
  • OSP antibody
  • OTM antibody
see all

Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker images:

  Immunohistochemistry - Free Floating - Oligodendrocyte Specific Protein OSP antibody - Oligodendrocyte Marker (ab7474)

Immunohistochemistry - Free Floating - Oligodendrocyte Specific Protein OSP antibody - Oligodendrocyte Marker (ab7474)

Immunofluorescent staining for OSP obtained with OSP antibody (ab7474) in rat brain cortex. The picture shows oligodendrocyte immunostaining in the cingular cortex. Picture taken with X20 objective, cells stained are ~30microns in diameter. Animals were intracardially perfused with 4% PFA. Tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. Protocol : free floating IHC on 30um cryostat sections. Primary antibody ab7474 was used at 1/100 and incubated overnight at RT. Secondary antibody, Alexa fluor 488 at 1/1000, incubated for 2h at RT.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker(ab7474)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker(ab7474)

IHC image of ab7474 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7474, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474)

This product has been referenced in:

  • McCabe MJet al. Gonadotropins regulate rat testicular tight junctions in vivo. Endocrinology 151:2911-22 (2010). WB; Rat.Read more (PubMed: 20357222) »
  • Simard JMet al. Brief suppression of Abcc8 prevents autodestruction of spinal cord after trauma. Sci Transl Med 2:28ra29 (2010).Read more (PubMed: 20410530) »

See all 4 publications for this product

Publishing research using ab7474? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"