Overview

  • Product name
    Anti-Oncostatin M antibody
    See all Oncostatin M primary antibodies
  • Description
    Rabbit polyclonal to Oncostatin M
  • Tested applications
    Suitable for: Indirect ELISA, Sandwich ELISA, WB, Neutralisingmore details
  • Species reactivity
    Reacts with: Recombinant fragment
    Predicted to work with: Human
  • Immunogen

    Highly pure (>98%) recombinant Human Oncostatin M.

  • Positive control
    • ab110621 was purified by affinity chromatography employing immobilized Human Oncostatin M matrix.

Properties

Applications

Our Abpromise guarantee covers the use of ab110621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Oncostatin M.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Oncostatin M (Biotin) (ab84256). Using ab84256 as the detection antibody and ab110621 as the capture antibody, the detection limit for recombinant Human Oncostatin M is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 28 kDa. The detection limit for recombinant Human Oncostatin M is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralising Use a concentration of 0.1 - 0.175 µg/ml. to yield one-half maximal inhibition [ND50] of the biological activity of Oncostatin M (10.0 ng/ml)

Target

  • Function
    Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST).
  • Sequence similarities
    Belongs to the LIF/OSM family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC20461 antibody
    • ONCM_HUMAN antibody
    • Oncostatin M antibody
    • Oncostatin-M antibody
    • OSM antibody
    see all

References

ab110621 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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