• Product nameAnti-ONECUT2 antibody
    See all ONECUT2 primary antibodies
  • Description
    Rabbit polyclonal to ONECUT2
  • Specificityab28466 recognises one cut domain family member 2 (ONECUT2)
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, Zebrafish
    Predicted to work with: Rat, Dog
  • Immunogen

    Synthetic peptide derived from residues 274 - 323 of human ONECUT2.


  • Positive control
    • Jurkat cell lysate. Human kidney



Our Abpromise guarantee covers the use of ab28466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.
IHC-P Use at an assay dependent concentration.


  • RelevanceONECUT2 is a member of the transcription factors of the ONECUT class, whose prototype is hepatocyte nuclear factor (HNF)-6. The distribution of OC-2 mRNA in humans is tissue-restricted, the strongest expression being detected in the liver and skin. ONECUT2 participates in the network of transcription factors required for liver differentiation and metabolism.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • HGNC:8139 antibody
    • MGC120377 antibody
    • MGC120378 antibody
    • OC 2 antibody
    • OC2 antibody
    • One cut domain family member 2 antibody
    • ONECUT 2 antibody
    • ONECUT 2 homeodomain transcription factor antibody
    • ONECUT 2 protein antibody
    • ONECUT2 homeodomain transcription factor antibody
    • ONECUT2 protein antibody
    • Transcription factor ONECUT 2 antibody
    • Transcription factor ONECUT2 antibody
    see all

Anti-ONECUT2 antibody images

  • Anti-ONECUT2 antibody (ab28466) at 1.25 µg/ml + Jurkat cell lysate

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • ONECUT2 expression detected in human kidney sections with 4-8ug/ml ab28466. The arrows indicate positive staining (epithelial cells of renal tubule). Magnification: 40X.
  • All lanes : Anti-ONECUT2 antibody (ab28466) at 1.25 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish liver homogenate (20ug)
    Lane 3 : Mouse liver homogenate (20ug)
    Lane 4 : JURKAT (Human T cell lymphoblast-like cell line) Whole Cell Lysate (20ug)

    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

    Exposure time : 1 minute

References for Anti-ONECUT2 antibody (ab28466)

This product has been referenced in:
  • Ao R  et al. Altered microRNA-9 Expression Level is Directly Correlated with Pathogenesis of Nonalcoholic Fatty Liver Disease by Targeting Onecut2 and SIRT1. Med Sci Monit 22:3804-3819 (2016). WB ; Human . Read more (PubMed: 27756894) »
  • Bhatnagar S  et al. Increased microRNA-34c abundance in Alzheimer's disease circulating blood plasma. Front Mol Neurosci 7:2 (2014). WB ; Human . Read more (PubMed: 24550773) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - Triton X-100
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde

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Submitted Jul 11 2016