• Product nameAnti-OPA1 antibody [1E81D9]
    See all OPA1 primary antibodies
  • Description
    Mouse monoclonal [1E81D9] to OPA1
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Human, full-length, corresponding to short form of isoform 1

  • General notes

    Product was previously marketed under the MitoSciences sub-brand.



Our Abpromise guarantee covers the use of ab119685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionDynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
  • Tissue specificityHighly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues.
  • Involvement in diseaseDefects in OPA1 are a cause of optic atrophy type 1 (OPA1) [MIM:165500]. OPA1 is a dominantly inherited optic neuropathy occurring in 1 in 50,000 individuals that features progressive loss in visual acuity leading, in many cases, to legal blindness.
    Defects in OPA1 are the cause of optic atrophy 1 with deafness (OPA1D) [MIM:125250]. Some individuals with mutations in OPA1 manifest also ophthalmoplegia and myopathy.
  • Sequence similaritiesBelongs to the dynamin family.
  • Post-translational
    PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion.
  • Cellular localizationMitochondrion inner membrane. Mitochondrion intermembrane space.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dynamin like 120 kDa protein antibody
    • Dynamin like 120 kDa protein, mitochondrial antibody
    • Dynamin-like 120 kDa protein, form S1 antibody
    • FLJ12460 antibody
    • Juvenile kjer type optic atrophy antibody
    • KIAA0567 antibody
    • KJER type antibody
    • Large GTP binding protein antibody
    • largeG antibody
    • MGM1 antibody
    • Mitochondrial dynamin like 120 kDa protein antibody
    • Mitochondrial dynamin like GTPase antibody
    • NPG antibody
    • NTG antibody
    • OAK antibody
    • OPA 1 antibody
    • opa1 antibody
    • OPA1 gene antibody
    • OPA1_HUMAN antibody
    • Optic atrophy 1 (autosomal dominant) antibody
    • OPTIC ATROPHY 1 antibody
    • Optic atrophy 1 gene protein antibody
    • Optic atrophy 1 homolog (human) antibody
    • Optic atrophy protein 1 antibody
    • Optic atrophy protein 1 homolog antibody
    see all

Anti-OPA1 antibody [1E81D9] images

  • Overlay histogram showing SH-SY5Y cells stained with ab119685 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119685, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-OPA1 antibody [1E81D9] (ab119685) at 1 µg/ml

    Lane 1 : whole cell lysates from HeLa cells(human)
    Lane 2 : whole cell lysates from H4IIE cells(rat)
    Lane 3 : whole cell lysates from MEF cells(mouse)

    Lysates/proteins at 30 µg per lane.

    HRP goat anti-mouse at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 112 kDa

References for Anti-OPA1 antibody [1E81D9] (ab119685)

This product has been referenced in:
  • Pfeffer G  et al. Mutations in the SPG7 gene cause chronic progressive external ophthalmoplegia through disordered mitochondrial DNA maintenance. Brain 137:1323-36 (2014). Read more (PubMed: 24727571) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (placenta)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 100 µg
Specification placenta
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Mrs. Polina Vishnyakova

Verified customer

Submitted Jun 21 2016

The epitope for this antibody hasn’t been mapped but the immunogen was the C-terminal region common to all of the OPA1 splice variants. Therefore, the antibody is expected to bind to all splice variants.

There are 8 different splice iso...

Read More