Overview

  • Product name
    Anti-OPALIN antibody
  • Description
    Rabbit polyclonal to OPALIN
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    amino acids 55-141 (RRRSSIEAME ESDRPCEISE IDDNPKISEN PRRSPTHEKN TMGAQEAHIY VKTVAGSEEP VHDRYRPTIE MERRRGLWWL VPRLSLE) of Human OPALIN.

  • Positive control
    • Human cerebellum tissue; Transfected HEK293T cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Tissue specificity
    Brain specific.
  • Cellular localization
    Cell membrane. In the CNS, enriched in the myelin paranodal and inner loop membranes, but not that of the PNS.
  • Information by UniProt
  • Database links
  • Alternative names
    • HTMP10 antibody
    • Oligodendrocytic myelin paranodal and inner loop protein antibody
    • OPALI_HUMAN antibody
    • OPALIN antibody
    • TMEM10 antibody
    • TMP10 antibody
    • Transmembrane protein 10 antibody
    • transmembrane protein TMP10 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hippocampus tissue labelling OPALIN with ab121425. Staining shows moderate cytoplasmic positivity in glial cells.
  • All lanes : Anti-OPALIN antibody (ab121425) at 1/250 dilution

    Lane 1 : Vector only transfected HEK293T cell lysate
    Lane 2 : Lysate from transfected HEK293T cells overexpressing OPALIN with a C-terminal myc-DDK tag (~3.1 kDa)

    Developed using the ECL technique

References

ab121425 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121425.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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