• Product name
    Anti-OPCML antibody
  • Description
    Rabbit polyclonal to OPCML
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 75-341 of Human OPCML (BC117254).

  • Positive control
    • WB: Human fetal brain lysate and HeLa cell lysate IHC-P: Human fetal brain tissue IF/ICC: PC12 cell line.



Our Abpromise guarantee covers the use of ab100923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 38 kDa.
IHC-P 1/100 - 1/500.


  • Function
    Binds opioids in the presence of acidic lipids; probably involved in cell contact.
  • Involvement in disease
    Ovarian cancer
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. IgLON family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GM181 antibody
    • IgLON family member 1 antibody
    • IGLON1 antibody
    • OBCAM antibody
    • OPCM antibody
    • OPCM_HUMAN antibody
    • OPCML antibody
    • Opiate binding cell adhesion molecule antibody
    • Opioid binding cell adhesion molecule antibody
    • Opioid binding protein/cell adhesion molecule antibody
    • Opioid binding protein/cell adhesion molecule-like antibody
    • Opioid binding protein/cell adhesion molecule-like preprotein antibody
    • Opioid-binding cell adhesion molecule antibody
    • Opioid-binding protein/cell adhesion molecule antibody
    see all


  • ICC/IF image of ab100923 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab100923, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM


  • All lanes : Anti-OPCML antibody (ab100923) at 1/500 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : HeLa cell lysate

    Predicted band size: 38 kDa

  • ab100923, at a 1/100 dilution, staining OPCML in formalin fixed, paraffin embedded Human fetal brain tissue by Immunohistochemistry.


This product has been referenced in:
  • Li C  et al. OPCML is frequently methylated in human colorectal cancer and its restored expression reverses EMT via downregulation of smad signaling. Am J Cancer Res 5:1635-48 (2015). Read more (PubMed: 26175934) »

See 1 Publication for this product

Customer reviews and Q&As

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