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Western blotting (WB) is a key technique in current life science research. By utilizing monoclonal or polyclonal antibodies to specific antigen proteins, it is possible to identify the signal of a protein of interest, quantitatively or qualitatively, on blotting membranes.
However, due to the complexities of biological materials and the insufficient specificity of some utilized antibodies, some intriguing WB results are generally observed, such as multiple band signals or hybridized bands with unclear molecular weights. To validate a WB result, calculating the molecular weight of the obtained protein signals may be the most straight forward means. Nevertheless, since most (pre-stained) molecular weight standard markers cannot be visualized after the hybridization procedure, many laboratories may align the WB result with the original membrane blot. Such procedure is tedious and may introduce estimation bias.
Optiblot Luminol Membrane Pen is designed to simplify such tasks and improve accuracy. After blotting proteins from gels to PVDF or nitrocellulose membranes, just use the Optiblot Luminol Membrane Pen to mark the (pre-stained) molecular weight standard markers and perform regular hybridization procedures. During the colorimetric or chemiluminenscent development step, the bands of molecular weight standard marker can be visualized and recorded for further analysis. Susceptible protein bands are easily located from the WB result. Additionally, in WB application, the Optiblot Luminol Membrane Pen can be used for blot annotation.
Our Abpromise guarantee covers the use of ab166858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
During the colorimetric or chemiluminenscent development step, the bands of molecular weight standard marker can be visualized and recorded for further analysis.
Susceptible protein bands are easily located from the WB result. (red arrow indicated). Additionally, in WB application, the Optiblot Luminol Membrane Pen can be used as personal noting or marking as positive control.
ab166858 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"