Optiblot Luminol Membrane Pen (ab166858)

Overview

  • Product name
    Optiblot Luminol Membrane Pen
  • Tested applications
    Suitable for: WBmore details
  • General notes

    Western blotting (WB) is a key technique in current life science research. By utilizing monoclonal or polyclonal antibodies to specific antigen proteins, it is possible to identify the signal of a protein of interest, quantitatively or qualitatively, on blotting membranes.

    However, due to the complexities of biological materials and the insufficient specificity of some utilized antibodies, some intriguing WB results are generally observed, such as multiple band signals or hybridized bands with unclear molecular weights. To validate a WB result, calculating the molecular weight of the obtained protein signals may be the most straight forward means. Nevertheless, since most (pre-stained) molecular weight standard markers cannot be visualized after the hybridization procedure, many laboratories may align the WB result with the original membrane blot. Such procedure is tedious and may introduce estimation bias.

    Optiblot Luminol Membrane Pen is designed to simplify such tasks and improve accuracy. After blotting proteins from gels to PVDF or nitrocellulose membranes, just use the Optiblot Luminol Membrane Pen to mark the (pre-stained) molecular weight standard markers and perform regular hybridization procedures. During the colorimetric or chemiluminenscent development step, the bands of molecular weight standard marker can be visualized and recorded for further analysis. Susceptible protein bands are easily located from the WB result. Additionally, in WB application, the Optiblot Luminol Membrane Pen can be used for blot annotation.

Properties

Applications

Our Abpromise guarantee covers the use of ab166858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

Images

  • During the colorimetric or chemiluminenscent development step, the bands of molecular weight standard marker can be visualized and recorded for further analysis.

    Susceptible protein bands are easily located from the WB result. (red arrow indicated). Additionally, in WB application, the Optiblot Luminol Membrane Pen can be used as personal noting or marking as positive control.

Protocols

References

ab166858 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

nice pen

Excellent Excellent 5/5 (Ease of Use)
Abreviews
The pen works well and is easy to handle.
The signal is strong and precise. We use the pen to label the bands of pre-stained molecular weight markers to make them visible in chemiluminescence.
Username

Abcam user community

Verified customer

Submitted Mar 06 2018


When the membrane needs to be exposed for a long time, we suggest drawing a weaker band as far as possible from the wells on the membrane with the luminol pen in order to avoid interference.

The LuminolPen can provide 1,000 membranes drawing.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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