Optiblot SDS Gel 4-20% (8x10cm) - 12 Well (ab119209)

Overview

  • Product nameOptiblot SDS Gel 4-20% (8x10cm) - 12 WellSee all SDS-PAGE Gel 4-20% (Triethanolamine) reagents ...
  • Tested applicationsWB, SDS-PAGEmore details
  • General notesOptiblot precast gels offer superior rigidity and stability over traditional polyacrlyamide gels and are compatible with the most common tanks and systems. For convenience the precast gels come with the comb already removed as the cassette locks the fingers in place; for additional convenience there is no tape to be removed and cassettes can be opened by hand.

    Optiblot gels must be used with Optiblot buffers.

    Learn more about Optiblot in our FAQs.

    Storage: For long term storage (up to 2 years) store at 4°C or for short-term storage (up to 6 months) store at room temperature.

    Number of Wells: 12

    Manufactured under patent number US 5,769.22

Properties

    Applications

    Our Abpromise guarantee covers the use of ab119209 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent concentration.
    SDS-PAGE Use at an assay dependent concentration.

    Optiblot SDS Gel 4-20% (8x10cm) - 12 Well images

    • Gel benefits - Click to expand



    • Migration Chart in kDa with SDS Buffer (ab119197)
    • Migration Chart in kDa with Reducing Buffer (ab119195) - Click to expand

    Protocols

    References for Optiblot SDS Gel 4-20% (8x10cm) - 12 Well (ab119209)

    ab119209 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.
    The lab let me know that using Laemmli buffer is possible but far from ideal. The best results will be obtained with LDS sample buffer composition (ab119196).
    I hope this information is helpful to you. Please do n...

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    Thank you for your reply.
    I'd suggest using the sample buffer that is compatible with the gel, namely ab119196.
    http://www.abcam.com/optiblot-lds-sample-buffer-4x-ab119196.html
    This is what is recommended to be used.
    I can doubleche...

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    Thank you for contacting us.
    Yes, the 8x10 Optiblot gels are compatible with the Bio-Rad Mini-PROTEAN tank.
    Please also check our FAQ section on Optiblots:
    http://www.abcam.com/index.html?pageconfig=resource&rid=14722
    where your que...

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    I have a question about your Optiblot pre-casted SDS gel products. How long can I use those SDS gels after purchasing?

    Thank you for your enquiry about Optiblot pre-casted SDS gels. We recommend long term storage of up to 24 months store at ...

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    Thank you for attending our WB Webinar.

    It is possible to cast your own gradient gels, but the process requires special equipment and is time consuming. If you would like to learn more about the protocol, see the following reference:
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    Thank you for contacting Abcam and we appreciate your interest in our Optiblot product range. Although not specified on our website, I believe that the Optiblot SDS Gels, 4-20% (8x10cm) have 12 wells and can hold a maximum of 35ul per well. I am in the...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"