• Product nameAnti-Optineurin antibody
    See all Optineurin primary antibodies
  • Description
    Mouse polyclonal to Optineurin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human Optineurin, amino acids 1-577 (NP_001008212.1)

  • Positive control
    • Human placenta tissue lysate, Optineurin transfected 293T cell lysate.



Our Abpromise guarantee covers the use of ab89820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa.


  • FunctionPlays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Negatively regulates the induction of IFNB in response to RNA virus infection. Plays a neuroprotective role in the eye and optic nerve. Probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). May constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death.
  • Tissue specificityPresent in acqueous humor of the eye (at protein level). Highly expressed in trabecular meshwork. Expressed nonpigmented ciliary epithelium, retina, brain, adrenal cortex, fetus, lymphocyte, fibroblast, skeletal muscle, heart, liver, brain and placenta.
  • Involvement in diseaseDefects in OPTN are the cause of primary open angle glaucoma type 1E (GLC1E) [MIM:137760]. Primary open angle glaucoma (POAG) is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place.
    Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG) [MIM:606657].
    Defects in OPTN are the cause of amyotrophic lateral sclerosis type 12 (ALS12) [MIM:613435]. It is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.
  • DomainUbiquitin-binding motif (UBAN) is essential for its inhibitory function, subcellular localization and interaction with TBK1.
  • Post-translational
    Phosphorylated. Phosphorylation is induced by phorbol esters and decreases its half-time.
  • Cellular localizationCytoplasm > perinuclear region. Golgi apparatus. Golgi apparatus > trans-Golgi network. Found in the perinuclear region and associates with the Golgi apparatus. Colocalizes with MYO6 and RAB8 at the Golgi complex and in vesicular structures close to the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14.7K interacting protein antibody
    • Ag9 C5 antibody
    • ALS12 antibody
    • E3 14.7K interacting protein antibody
    • E3-14.7K-interacting protein antibody
    • FIP 2 antibody
    • FIP-2 antibody
    • FIP2 antibody
    • Glaucoma 1 open angle E (adult onset) antibody
    • Glaucoma 1 open angle E antibody
    • GLC1E antibody
    • HIP 7 antibody
    • HIP-7 antibody
    • HIP7 antibody
    • Huntingtin interacting protein 7 antibody
    • Huntingtin interacting protein HYPL antibody
    • Huntingtin interacting protein L antibody
    • Huntingtin yeast partner L antibody
    • Huntingtin-interacting protein 7 antibody
    • Huntingtin-interacting protein L antibody
    • HYPL antibody
    • Injury inducible protein I 55 antibody
    • NEMO related protein antibody
    • NEMO-related protein antibody
    • NRP antibody
    • Optic neuropathy inducing protein antibody
    • Optic neuropathy-inducing protein antibody
    • Optineurin antibody
    • OPTN antibody
    • OPTN_HUMAN antibody
    • TFIIIA IntP antibody
    • TFIIIA-IntP antibody
    • Transcription factor IIIA interacting protein antibody
    • Transcription factor IIIA-interacting protein antibody
    • Tumor necrosis factor alpha inducible cellular protein containing leucine zipper domains antibody
    see all

Anti-Optineurin antibody images

  • Anti-Optineurin antibody (ab89820) at 1 µg/ml + Human placenta tissue lysate at 50 µg

    Predicted band size : 66 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Optineurin antibody (ab89820) at 1 µg/ml

    Lane 1 : Optineurin transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size : 66 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

References for Anti-Optineurin antibody (ab89820)

This product has been referenced in:
  • Kumar DM  et al. Temozolomide-modulated glioma proteome: Role of interleukin-1 receptor-associated kinase-4 (IRAK4) in chemosensitivity. Proteomics 13:2113-24 (2013). Human . Read more (PubMed: 23595970) »

See 1 Publication for this product

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