Overview

  • Product name
    Anti-OR3A4 antibody
  • Description
    Mouse polyclonal to OR3A4
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length OR3A4 protein (Human)

  • Positive control
    • 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 37 kDa).

Target

  • Relevance
    Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G protein coupled receptors (GPCR) arising from single coding exon genes. Olfactory receptors share a 7 transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms.
  • Cellular localization
    Cell membrane; Multipass membrane protein
  • Database links
  • Alternative names
    • OLFRA05 antibody
    • OLFRA06 antibody
    • OR17 13 antibody
    • OR17 16 antibody
    • OR17 24 antibody
    • OR17 25 antibody
    • OR24 antibody
    • OR25 antibody
    • OR3A4P antibody
    • OR3A5P antibody
    see all

Images

  • All lanes : Anti-OR3A4 antibody (ab67107) at 1/500 dilution

    Lane 1 : OR3A4 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg/ml per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 37 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

References

ab67107 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH9 (ER2) 20 mins
Permeabilization
No
Specification
Colon
Blocking step
H202 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up