The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: 1/1000 - 1/2500.
IP: Use at 1µg.
WB: 1/1000 - 1/5000. Detects a band of approximately 50 and 35-50 kDa (predicted molecular weight: 33 kDa). By Western blot the antibody reacts with the ~50kDa Orai1 protein. Additional bands between 35 and 50kDa may be seen if analyzing over-expressed Orai1. These additional bands most likely represent different degrees of glycosylation of Orai1.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCa(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.
Involvement in diseaseDefects in ORAI1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1) [MIM:612782]. IDTICED1 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, and normal lymphocytes counts and serum immunoglobulin levels. In surviving patients ectodermal dysplasia with anhydrosis and non-progressive myopathy may be observed.
Yang B & Bouchard MJ The hepatitis B virus X protein elevates cytosolic calcium signals by modulating mitochondrial calcium uptake. J Virol86:313-27 (2012).
Read more (PubMed: 22031934) »
Khadra N et al. CD95 triggers Orai1-mediated localized Ca2+ entry, regulates recruitment of protein kinase C (PKC) ß2, and prevents death-inducing signaling complex formation. Proc Natl Acad Sci U S A108:19072-7 (2011).
Read more (PubMed: 22065776) »