• Product name
  • Description
    Rabbit polyclonal to Orai1
  • Specificity
    Detects both the glycosylated and un-glycosylated forms of Orai1.
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:


    , corresponding to intracellular amino acids 22-40 of Human Orai1

  • Positive control
    • Cell membrane preparations containing protease inhibitors. Jurkat whole cell lysates.



Our Abpromise guarantee covers the use of ab78471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ICC/IF: 1/1000 - 1/2500.
    IP: Use at 1µg.
    WB: 1/1000 - 1/5000. Detects a band of approximately 50 and 35-50 kDa (predicted molecular weight: 33 kDa). By Western blot the antibody reacts with the ~50kDa Orai1 protein. Additional bands between 35 and 50kDa may be seen if analyzing over-expressed Orai1. These additional bands most likely represent different degrees of glycosylation of Orai1.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.
    • Involvement in disease
      Defects in ORAI1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1) [MIM:612782]. IDTICED1 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, and normal lymphocytes counts and serum immunoglobulin levels. In surviving patients ectodermal dysplasia with anhydrosis and non-progressive myopathy may be observed.
    • Sequence similarities
      Belongs to the Orai family.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Calcium release activated calcium channel protein 1 antibody
      • Calcium release activated calcium modulator 1 antibody
      • Calcium release-activated calcium channel protein 1 antibody
      • CRACM 1 antibody
      • CRACM1 antibody
      • CRCM1 antibody
      • CRCM1_HUMAN antibody
      • FLJ14466 antibody
      • Orai 1 antibody
      • ORAI calcium release activated calcium modulator 1 antibody
      • orai1 antibody
      • ORAT1 antibody
      • Protein orai 1 antibody
      • Protein orai-1 antibody
      • TMEM 142A antibody
      • TMEM142A antibody
      • Transmembrane protein 142A antibody
      see all

    Anti-Orai1 antibody images

    • Lane 1 : Anti-Orai1 antibody (ab78471) at 0.5 µg/ml
      Lane 2 : Anti-Orai1 antibody (ab78471) at 1 µg/ml
      Lane 3 : Anti-Orai1 antibody (ab78471) at 5 µg/ml

      Lane 1 : Jurkat cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 50 µg per lane.

      HRP-conjugated goat anti-rabbit

      Predicted band size : 33 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 35-50 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 1 minute

    References for Anti-Orai1 antibody (ab78471)

    This product has been referenced in:
    • Yang B & Bouchard MJ The hepatitis B virus X protein elevates cytosolic calcium signals by modulating mitochondrial calcium uptake. J Virol 86:313-27 (2012). WB . Read more (PubMed: 22031934) »
    • Khadra N  et al. CD95 triggers Orai1-mediated localized Ca2+ entry, regulates recruitment of protein kinase C (PKC) ß2, and prevents death-inducing signaling complex formation. Proc Natl Acad Sci U S A 108:19072-7 (2011). Read more (PubMed: 22065776) »

    See all 2 Publications for this product

    Product Wall

    Western blot
    Human Cell lysate - other (Jurkat T cells)
    Loading amount
    40 µg
    Jurkat T cells
    Gel Running Conditions
    Reduced Denaturing (10%/5% stacking)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Dr. Barbara Niemeyer

    Verified customer

    Submitted Jun 10 2010


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