Overview

  • Product nameAnti-Orai1 antibody
    See all Orai1 primary antibodies
  • Description
    Rabbit polyclonal to Orai1
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from the center region of Human Orai1 (NP_116179.2), conjugated to KLH.

  • Positive control
    • A375 cell lysate, Mouse testis tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/1000. Predicted molecular weight: 33 kDa.

Target

  • FunctionCa(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.
  • Involvement in diseaseDefects in ORAI1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1) [MIM:612782]. IDTICED1 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, and normal lymphocytes counts and serum immunoglobulin levels. In surviving patients ectodermal dysplasia with anhydrosis and non-progressive myopathy may be observed.
  • Sequence similaritiesBelongs to the Orai family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium release activated calcium channel protein 1 antibody
    • Calcium release activated calcium modulator 1 antibody
    • Calcium release-activated calcium channel protein 1 antibody
    • CRACM 1 antibody
    • CRACM1 antibody
    • CRCM1 antibody
    • CRCM1_HUMAN antibody
    • FLJ14466 antibody
    • Orai 1 antibody
    • ORAI calcium release activated calcium modulator 1 antibody
    • orai1 antibody
    • ORAT1 antibody
    • Protein orai 1 antibody
    • Protein orai-1 antibody
    • TMEM 142A antibody
    • TMEM142A antibody
    • Transmembrane protein 142A antibody
    see all

Anti-Orai1 antibody images

  • All lanes : Anti-Orai1 antibody (ab86748) at 1/1000 dilution

    Lane 1 : A375 cell lysate
    Lane 2 : Mouse testis tissue lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 33 kDa
  • Immunohistochemical staining of formalin-fixed and paraffin-embedded human prostate carcinoma with ab86748 followed by a HRP-conjugated secondary antibody and DAB staining.

References for Anti-Orai1 antibody (ab86748)

ab86748 has not yet been referenced specifically in any publications.

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Western blotting is a technique that separates proteins based on size. In general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other fact...

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