Overview

  • Product nameAnti-Orai2 antibody
    See all Orai2 primary antibodies
  • Description
    Rabbit polyclonal to Orai2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Cat, Dog
  • Immunogen

    A synthetic peptide corresponding to a region of Human Orai2

  • Positive control
    • MCF7 cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab75500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

Target

  • FunctionCa(2+) release-activated Ca(2+)-like (CRAC-like) channel subunit which mediates Ca(2+) influx and increase in Ca(2+)-selective current by synergy with the Ca(2+) sensor, STIM1.
  • Sequence similaritiesBelongs to the Orai family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C7orf19 antibody
    • CAP binding protein complex interacting protein 2 antibody
    • CAP-binding protein complex-interacting protein 2 antibody
    • CBCIP 2 antibody
    • CBCIP2 antibody
    • Chromosome 7 open reading frame 19 antibody
    • FLJ12474 antibody
    • FLJ14733 antibody
    • FLJ44818 antibody
    • H_NH0514P08.8 antibody
    • Orai 2 antibody
    • ORAI calcium release activated calcium modulator 2 antibody
    • ORAI2 antibody
    • ORAI2_HUMAN antibody
    • Protein orai 2 antibody
    • Protein orai-2 antibody
    • TMEM 142B antibody
    • TMEM142B antibody
    • Transmembrane protein 142B antibody
    see all

Anti-Orai2 antibody images

  • Anti-Orai2 antibody (ab75500) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 29 kDa
    Observed band size : 29 kDa

References for Anti-Orai2 antibody (ab75500)

ab75500 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 50 µg
Specification HEK293
Treatment Transfected with Orai2-expression vecter
Gel Running Conditions Reduced Denaturing (AnyKD gel (biorad))
Blocking step Immunoblock as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 23 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"