• Product nameAnti-ORC1 antibody
    See all ORC1 primary antibodies
  • Description
    Rabbit polyclonal to ORC1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 811 and 861 of human ORC1 (NP_004144.2)

  • Positive control
    • HeLa and 293T whole cell lysates.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab85830 was affinity purified using an epitope specific to ORC1 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab85830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
IP Use at 2-5 µg/mg of lysate.


  • FunctionComponent of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
  • Sequence similaritiesBelongs to the ORC1 family.
    Contains 1 BAH domain.
  • Developmental stageExpression is cell-cycle regulated, it starts to accumulate in mid-G1 phase, reaches a peak at the G1/S boundary, and decreases to a basal level in S phase (at protein level).
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HSORC1 antibody
    • MmORC1 antibody
    • orc1 antibody
    • ORC1_HUMAN antibody
    • ORC1L antibody
    • Origin Recognition Complex 1 antibody
    • Origin recognition complex subunit 1 (yeast homolog) like antibody
    • Origin recognition complex subunit 1 antibody
    • Origin recognition complex subunit 1 homolog antibody
    • Origin recognition complex subunit 1 like (S. cerevisia antibody
    • Origin recognition complex subunit 1 like antibody
    • Origin recognition complex subunit 1 S. cerevisiae homolog like antibody
    • Origin recognition complex, subunit 1 like (yeast) antibody
    • PARC1 antibody
    • Replication control protein 1 antibody
    see all

Anti-ORC1 antibody images

  • All lanes : Anti-ORC1 antibody (ab85830) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size : 97 kDa
    Observed band size : 107 kDa (why is the actual band size different from the predicted?)

    Exposure time : 3 minutes
  • Detection of Human ORC1 by Western Blot of Immunprecipitate.
    All lanes: ab85830 at 1µg/ml staining ORC1 in HeLa whole cell lysates immunoprecipitated at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) using the following antibodies:
    Lane 1: ab85830
    Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-ORC1 antibody (ab85830)

This product has been referenced in:
  • Maya-Mendoza A  et al. Immortalised breast epithelia survive prolonged DNA replication stress and return to cycle from a senescent-like state. Cell Death Dis 5:e1351 (2014). WB ; Human . Read more (PubMed: 25058425) »
  • Chan KM & Zhang Z Leucine-rich Repeat and WD Repeat-containing Protein 1 Is Recruited to Pericentric Heterochromatin by Trimethylated Lysine 9 of Histone H3 and Maintains Heterochromatin Silencing. J Biol Chem 287:15024-33 (2012). WB . Read more (PubMed: 22427655) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.

The discount code ****** is unique and specific for the test of ab85830 in Xenopus;it is not valid for the test of another antibody.

Furthermore, as shown on the product datasheet, the anti-histone H3 http...

Read More

ab31506 : ******
ab85830 : ******
Expiration date: DD MM YYYY.

I am very pleased to hear you would like to accept our offer and test ab31506 and ab85830 in Xenopus. Each code will give you1 freeprimary antibodybefor...

Read More