Overview

  • Product name
    Anti-ORC1 antibody [TK 1/2]
    See all ORC1 primary antibodies
  • Description
    Mouse monoclonal [TK 1/2] to ORC1
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Xenopus laevis
    Does not react with: a wide range of other species
  • Immunogen

    Recombinant full length protein (Xenopus laevis).

  • Epitope
    The epitope is within the first 280 residues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    TK 1/2
  • Myeloma
    Sp2/0-Ag14
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Detects a band of approximately 105 kDa (predicted molecular weight: 97 kDa).

Target

  • Function
    Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
  • Sequence similarities
    Belongs to the ORC1 family.
    Contains 1 BAH domain.
  • Developmental stage
    Expression is cell-cycle regulated, it starts to accumulate in mid-G1 phase, reaches a peak at the G1/S boundary, and decreases to a basal level in S phase (at protein level).
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Alternative names
    • HSORC1 antibody
    • MmORC1 antibody
    • orc1 antibody
    • ORC1_HUMAN antibody
    • ORC1L antibody
    • Origin Recognition Complex 1 antibody
    • Origin recognition complex subunit 1 (yeast homolog) like antibody
    • Origin recognition complex subunit 1 antibody
    • Origin recognition complex subunit 1 homolog antibody
    • Origin recognition complex subunit 1 like (S. cerevisia antibody
    • Origin recognition complex subunit 1 like antibody
    • Origin recognition complex subunit 1 S. cerevisiae homolog like antibody
    • Origin recognition complex, subunit 1 like (yeast) antibody
    • PARC1 antibody
    • Replication control protein 1 antibody
    see all

References

This product has been referenced in:
  • Tugal T  et al. The Orc4p and Orc5p subunits of the Xenopus and human origin recognition complex are related to Orc1p and Cdc6p. J Biol Chem 273:32421-9 (1998). Read more (PubMed: 9829972) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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