The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 21029866
1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 66 kDa).
Use a concentration of 5 µg/ml.
Use at an assay dependent concentration.
FunctionComponent of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.