Overview

  • Product nameAnti-ORC2 antibody
    See all ORC2 primary antibodies
  • Description
    Mouse monoclonal to ORC2
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length ORC2 protein (Human), with a tag. MW of the tag alone is 26kDa.

  • Positive control
    • Jurkat cell lysate and human spleen tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab68348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21029866
WB 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 66 kDa).
IHC-P Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent concentration.

Target

  • FunctionComponent of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
  • Sequence similaritiesBelongs to the ORC2 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • orc2 antibody
    • ORC2_HUMAN antibody
    • ORC2L antibody
    • Origin of replication 2 (yeast homolog) like antibody
    • Origin recognition complex protein 2 homolog antibody
    • Origin recognition complex subunit 2 antibody
    • Origin recognition complex subunit 2 isoform B antibody
    • Origin recognition complex subunit 2 like (yeast) antibody
    • Origin recognition complex, subunit 2 homolog antibody
    • Origin recognition complex, subunit 2, S. cerevisiae, homolog of antibody
    • Origin recognition complex, subunit 2-like (S. cerevisiae) antibody
    see all

Anti-ORC2 antibody images

  • Anti-ORC2 antibody (ab68348) at 1/500 dilution + Jurkat cell lysate at 25 µg/ml

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 66 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  • Immunoperoxidase of ab68348 on formalin-fixed paraffin-embedded human spleen tissue, antibody concentration at 5µg/ml.

References for Anti-ORC2 antibody (ab68348)

This product has been referenced in:
  • Ramos AC  et al. The kin17 Protein in Murine Melanoma Cells. Int J Mol Sci 16:27912-20 (2015). WB . Read more (PubMed: 26610484) »
  • Qattan AT  et al. Spatial Distribution of Cellular Function: The Partitioning of Proteins between Mitochondria and the Nucleus in MCF7 Breast Cancer Cells. J Proteome Res : (2012). Read more (PubMed: 23051583) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"