• Product nameAnti-ORC2 antibody
    See all ORC2 primary antibodies
  • Description
    Rabbit polyclonal to ORC2
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1-50 (MSKPELKEDK MLEVHFVGDD DVLNHILDRE GGAKLKKERA QLLVNPKKII) of Human ORC2 (NP_006181.1).

  • Positive control
    • Hela and 293T whole cell lysates



Our Abpromise guarantee covers the use of ab99277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionComponent of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
  • Sequence similaritiesBelongs to the ORC2 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • orc2 antibody
    • ORC2_HUMAN antibody
    • ORC2L antibody
    • Origin of replication 2 (yeast homolog) like antibody
    • Origin recognition complex protein 2 homolog antibody
    • Origin recognition complex subunit 2 antibody
    • Origin recognition complex subunit 2 isoform B antibody
    • Origin recognition complex subunit 2 like (yeast) antibody
    • Origin recognition complex, subunit 2 homolog antibody
    • Origin recognition complex, subunit 2, S. cerevisiae, homolog of antibody
    • Origin recognition complex, subunit 2-like (S. cerevisiae) antibody
    see all

Anti-ORC2 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ORC2 with ab99277 at 1/1000 (0.2µg/mg). Detection: DAB.
  • All lanes : Anti-ORC2 antibody (ab99277) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 66 kDa

    Exposure time : 30 seconds
  • Detection of ORC2 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab99277 at 3 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

References for Anti-ORC2 antibody (ab99277)

ab99277 has not yet been referenced specifically in any publications.

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