The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 2 µg/ml. Predicted molecular weight: 66 kDa.
Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionComponent of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
Origin recognition complex, subunit 2, S. cerevisiae, homolog of antibody
Origin recognition complex, subunit 2-like (S. cerevisiae) antibody
Anti-ORC2 antibody [SB46] images
Western blot - ORC2 antibody [SB46] (ab31930)
Predicted band size : 66 kDa
Image from Granata M et al, PLoS Genet. 2010 Aug 5;6(8). pii: e1001047, Fig S1.
Flow Cytometry-Anti-ORC2 antibody [SB46](ab31930)
Overlay histogram showing HeLa cells stained with ab31930 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab31930, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-ORC2 antibody [SB46] (ab31930)
This product has been referenced in:
Granata M et al. Dynamics of Rad9 chromatin binding and checkpoint function are mediated by its dimerization and are cell cycle-regulated by CDK1 activity. PLoS Genet6: (2010).
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