The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 82 kDa).Can be blocked with Human ORC3L peptide (ab23155). Please note that we also detect a band at approximately 35kDa in addition to the predicted band. This could be cell/tissue specific. For example, Thome et al., [J. Biol. Chem, Nov 2000; 275: 35233 - 35241] observed that "brain expressed a 40-45-kDa protein that reacted strongly with the anti-Orc3 antibody". Moreover, splice prediction software (http://www.bioinformatics.ucla.edu/ASAP/) indicated the possibility of a shorter isoform.
FunctionComponent of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
Sequence similaritiesBelongs to the ORC3 family.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated upon DNA damage, probably by ATM or ATR.