Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 82 kDa).Can be blocked with Human ORC3L peptide (ab23155).
Please note that we also detect a band at approximately 35kDa in addition to the predicted band. This could be cell/tissue specific. For example, Thome et al., [J. Biol. Chem, Nov 2000; 275: 35233 - 35241] observed that "brain expressed a 40-45-kDa protein that reacted strongly with the anti-Orc3 antibody". Moreover, splice prediction software (http://www.bioinformatics.ucla.edu/ASAP/) indicated the possibility of a shorter isoform.

Target

  • Function
    Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
  • Sequence similarities
    Belongs to the ORC3 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homolog of Latheo antibody
    • homolog of latheo, Drosophila antibody
    • LAT antibody
    • LATHEO antibody
    • LATHEO, drosophila, homolog of antibody
    • orc3 antibody
    • ORC3_HUMAN antibody
    • ORC3L antibody
    • Origin recognition complex subunit 3 antibody
    • Origin recognition complex subunit 3 honolog antibody
    • Origin Recognition Complex Subunit 3 Isoform 1 antibody
    • Origin recognition complex subunit 3 like antibody
    • Origin recognition complex subunit Latheo antibody
    • Origin Recognition Complex, subunit 3, S. cerevisiae, homolog of antibody
    • Origin recognition complex, subunit 3-like (S. cerevisiae) antibody
    • Origin recognition complex, subunit 3-like (yeast) antibody
    see all

Images



  • Predicted band size : 82 kDa


    Goat polyclonal to ORC3L (2µg/ml) staining of Hela lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.

References

This product has been referenced in:

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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