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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Purity Immunogen affinity purified
Purification notes Affinity purified using an epitope specific to ORC3L immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Predicted molecular weight: 82 kDa.
Application notes Is unsuitable for IP.
Function Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
Sequence similarities Belongs to the ORC3 family.
Post-translational modifications Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization Nucleus.
Information by UniProt
Homolog of Latheo antibody
homolog of latheo, Drosophila antibody
Anti-ORC3L antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORC3L antibody (ab99278)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling ORC3L with ab99278 at 1/200 (1µg/mg). Detection: DAB.
Western blot - ORC3L antibody (ab99278)
All lanes : Anti-ORC3L antibody (ab99278) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg developed using the ECL technique Predicted band size : 82 kDa Exposure time : 30 seconds
References for Anti-ORC3L antibody (ab99278)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"