Overview

  • Product name
  • Description
    Rabbit polyclonal to ORC3L
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within the internal sequence amino acids 325-375 of Human ORC3L (NP_036513.2).

  • Positive control
    • HeLa whole cell lysate and 293T cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity purified using an epitope specific to ORC3L immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 82 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
    • Sequence similarities
      Belongs to the ORC3 family.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Homolog of Latheo antibody
      • homolog of latheo, Drosophila antibody
      • LAT antibody
      • LATHEO antibody
      • LATHEO, drosophila, homolog of antibody
      • orc3 antibody
      • ORC3_HUMAN antibody
      • ORC3L antibody
      • Origin recognition complex subunit 3 antibody
      • Origin recognition complex subunit 3 honolog antibody
      • Origin Recognition Complex Subunit 3 Isoform 1 antibody
      • Origin recognition complex subunit 3 like antibody
      • Origin recognition complex subunit Latheo antibody
      • Origin Recognition Complex, subunit 3, S. cerevisiae, homolog of antibody
      • Origin recognition complex, subunit 3-like (S. cerevisiae) antibody
      • Origin recognition complex, subunit 3-like (yeast) antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling ORC3L with ab99278 at 1/200 (1µg/mg). Detection: DAB.
    • All lanes : Anti-ORC3L antibody (ab99278) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 82 kDa


      Exposure time : 30 seconds

    References

    ab99278 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab99278.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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