Overview

  • Product name
    Anti-ORC4L antibody - ChIP Grade
    See all ORC4L primary antibodies
  • Description
    Goat polyclonal to ORC4L - ChIP Grade
  • Tested applications
    Suitable for: ChIP, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide: DVRQWATSSLSWL, corresponding to C terminal amino acids 424-436 of Human ORC4L.

  • Positive control
    • Jurkat and NSO (mouse) cell lysates.
  • General notes
    Official Gene Symbol - ORC4L GenBank Accession Number – NP_002543 LocusLink ID - 5000

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human ORC4L peptide (ab23156).
ELISA Use at an assay dependent concentration.

Target

  • Function
    Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
  • Involvement in disease
    Defects in ORC4 are the cause of Meier-Gorlin syndrome type 2 (MGORS2) [MIM:613800]. MGORS2 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. Additional clinical findings include anomalies of cranial sutures, microcephaly, apparently low-set and simple ears, microstomia, full lips, highly arched or cleft palate, micrognathia, genitourinary tract anomalies, and various skeletal anomalies. While almost all cases have primordial dwarfism with substantial prenatal and postnatal growth retardation, not all cases have microcephaly, and microtia and absent/hypoplastic patella are absent in some. Despite the presence of microcephaly, intellect is usually normal.
  • Sequence similarities
    Belongs to the ORC4 family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Origin recognition complex, subunit 4, S. cerevisiae, homolog of antibody
    • FLJ46668 antibody
    • HSORC4 antibody
    • ORC 4 antibody
    • ORC 4L antibody
    • ORC 4P antibody
    • ORC4 antibody
    • ORC4_HUMAN antibody
    • ORC4L antibody
    • ORC4L protein antibody
    • ORC4P antibody
    • Origin recognition complex subunit 4 (yeast homolog) like antibody
    • Origin recognition complex subunit 4 antibody
    • Origin recognition complex subunit 4 like (yeast) antibody
    • Origin recognition complex subunit 4 like antibody
    • origin recognition complex, subunit 4 homolog antibody
    • Origin recognition complex, subunit 4, S. cerevisiae, homolog-like antibody
    see all

Anti-ORC4L antibody - ChIP Grade images

  • Anti-ORC4L antibody - ChIP Grade (ab9641) at 0.5 µg/ml + Mouse heart lysate at 35 µg

    Predicted band size : 50 kDa
  • Anti-ORC4L antibody - ChIP Grade (ab9641) at 0.5 µg/ml + Rat Heart lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 50 kDa
    Observed band size : 50 kDa


  • Predicted band size : 50 kDa
    ab9641 (2µg/ml) staining of Jurkat lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.
  • All lanes : Anti-ORC4L antibody - ChIP Grade (ab9641) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-ORC4L antibody - ChIP Grade (ab9641)

This product has been referenced in:
  • Hossain M & Stillman B Opposing roles for DNA replication initiator proteins ORC1 and CDC6 in control of Cyclin E gene transcription. Elife 5:N/A (2016). IP ; Human . Read more (PubMed: 27458800) »
  • Zhang R  et al. Targeting GLI by GANT61 involves mechanisms dependent on inhibition of both transcription and DNA licensing. Oncotarget 7:80190-80207 (2016). ICC/IF ; Human . Read more (PubMed: 27863397) »

See all 8 Publications for this product

Product Wall

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. This antibody was assigned "ChIP grade" status following its application in the following publication; Todorovic V et al. Human origins of ...

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Thank you for your enquiry. Unfortunately, lot #35325 is no longer in stock. Please rest assured, however, that we guarantee that ab9641 will work as stated on the online datasheet or we will provide a replacement or refund. Please contact us again...

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Thank you for your e-mail. I'm really sorry to hear you are having problems with this antibody and would like to give you a few suggestions. You have not mentioned the amount of protein loaded onto the gel. If it is under 30-40ug this may be too low...

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