ORC4L overexpression 293T lysate (whole cell) (ab94143)

Overview

  • Product nameORC4L overexpression 293T lysate (whole cell)
  • Description
    ORC4L overexpression lysate
  • General notesab94143 is a 293T cell transfected lysate in which Human ORC4L has been transiently over-expressed using a pCMV-ORC4L plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage bufferpH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Research areas
  • BackgroundFunction: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Disease: Defects in ORC4 are the cause of Meier-Gorlin syndrome type 2 (MGORS2) [MIM:613800]. MGORS2 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. Additional clinical findings include anomalies of cranial sutures, microcephaly, apparently low-set and simple ears, microstomia, full lips, highly arched or cleft palate, micrognathia, genitourinary tract anomalies, and various skeletal anomalies. While almost all cases have primordial dwarfism with substantial prenatal and postnatal growth retardation, not all cases have microcephaly, and microtia and absent/hypoplastic patella are absent in some. Despite the presence of microcephaly, intellect is usually normal. Similarity: Belongs to the ORC4 family.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

ORC4L overexpression 293T lysate (whole cell) images

  • ab94143 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-ORC4L antibody (ab88999) at 1/500 dilution

    Lane 1 : ORC4L overexpression 293T lysate (whole cell) (ab94143)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for ORC4L overexpression 293T lysate (whole cell) (ab94143)

ab94143 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Questions for ab94143.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"