Overview

  • Product nameAnti-ORC5L antibody
  • Description
    Rabbit polyclonal to ORC5L
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: GEASERDTRK LWRNIEPHLK KAMQTVYLRE ISSSQWEKLQ KDDTDPGQLK GLSAHTHVEL PYYSKFILIA A, corresponding to internal sequence amino acids 243-313 of Human ORC5L.

  • Positive control
    • Human kidney tissue; Human cell line U 2 OS.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab121610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. using Fixation/Permeabilization: PFA/Triton X-100.

Target

  • FunctionComponent of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
  • Sequence similaritiesBelongs to the ORC5 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ORC5 antibody
    • ORC5_HUMAN antibody
    • ORC5L antibody
    • ORC5P antibody
    • ORC5T antibody
    • Origin recognition complex subunit 5 antibody
    • Origin recognition complex subunit 5 like antibody
    see all

Anti-ORC5L antibody images

  • ab121610 at 1/100 dilution staining ORC5L in Paraffin-embedded Human kidney tissue by Immunohistochemistry.
    Note: strong nuclear and cytoplasmic positivity in renal tubules.
  • ab121610 at 4 µg/ml staining ORC5L in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).
    Note: ORC5

References for Anti-ORC5L antibody (ab121610)

ab121610 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"