Overview

  • Product name
    Anti-ORC6 antibody - C-terminal
  • Description
    Rabbit polyclonal to ORC6 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 191-252 of Human ORC6 (Uniprot ID: Q9Y5N6).

  • Positive control
    • 293T, MOLT4 and Raji whole cell lysates; Human TOV-21G xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 28 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. ORC6L is a subunit of the ORC complex. It has been shown that ORC6L and and ORC1L are loosely associated with the core complex consisting of ORC2L, 3L, 4L and 5L. Gene silencing studies with small interfering RNA demonstrated that ORC6L plays an essential role in coordinating chromosome replication and segregation with cytokinesis.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • ORC6L antibody
    • origin recognition complex subunit 6 antibody
    • origin recognition complex subunit 6 homolog like (yeast) antibody

Images

  • Anti-ORC6 antibody - C-terminal (ab153993) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 28 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human TOV-21G xenograft tissue labeling ORC6 with ab153993 at 1/500 dilution.

References

ab153993 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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