Anti-Orexin A antibody (ab35337)
Key features and details
- Sheep polyclonal to Orexin A
- Suitable for: IHC-FoFr
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Orexin A antibody
See all Orexin A primary antibodies -
Description
Sheep polyclonal to Orexin A -
Host species
Sheep -
Tested applications
Suitable for: IHC-FoFrmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human Orexin A aa 35-47 conjugated to Keyhole Limpet Haemocyanin (KLH). corresponding to amino acids 2-14 of human orexin A peptide
Sequence:NLPSTKVSWAAVT
Database link: O43612 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
IgG fraction -
Purification notes
Ammonium Sulfate fractionation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab35337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-FoFr |
Use at an assay dependent concentration.
|
Notes |
---|
IHC-FoFr
Use at an assay dependent concentration. |
Target
-
Relevance
Orexin A (hypocretin 1) a 33 amino acid peptide and orexin B (hypocretin 2), a 28 amino acid peptide, are both derived from a common 130 amino acid precursor, prepro orexin. Orexin A and Orexin B stimulate food consumption when administered intracerebroventricularly to rats. Orexin gene expression in the brain is highly restricted to distinct populations of neurons located in specific hypothalamic regions, including the lateral hypothalamic area (LHA), a region implicated in feeding behaviour. Orexin A and orexin B bind to and activate two closely related G protein coupled receptors (GPCRs), termed Orexin 1 (OX1) and Orexin 2 (OX2) receptors. Dysfunction of the orexin peptide system has been linked to narcolepsy. -
Cellular localization
Rough endoplasmic reticulum. Cytoplasmic vesicle. Cell junction, synapse. Note: Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses. -
Database links
- Entrez Gene: 3060 Human
- Entrez Gene: 25723 Rat
- Omim: 602358 Human
- SwissProt: O43612 Human
- SwissProt: O55232 Rat
-
Alternative names
- HCRT antibody
- HCRT1 antibody
- HCRT2 antibody
see all
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab35337 has been referenced in 1 publication.
- Silva JP et al. Regulation of adaptive behaviour during fasting by hypothalamic Foxa2. Nature 462:646-50 (2009). IHC-FoFr ; Mouse . PubMed: 19956259