Overview

  • Product nameAnti-Orexin A antibody
    See all Orexin A primary antibodies
  • Description
    Sheep polyclonal to Orexin A
  • SpecificityThis antibody is specific for Orexin A.
  • Tested applicationsSuitable for: IHC-FoFr, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human Orexin A aa 35-47 conjugated to Keyhole Limpet Haemocyanin (KLH). corresponding to amino acids 2-14 of human orexin A peptide
    Sequence:

    NLPSTKVSWAAVT


    Database link: O43612

Properties

Applications

Our Abpromise guarantee covers the use of ab35337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent dilution.
ELISA 1/200 - 1/800.
IHC-P 1/1000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

Target

  • RelevanceOrexin A (hypocretin 1) a 33 amino acid peptide and orexin B (hypocretin 2), a 28 amino acid peptide, are both derived from a common 130 amino acid precursor, prepro orexin. Orexin A and Orexin B stimulate food consumption when administered intracerebroventricularly to rats. Orexin gene expression in the brain is highly restricted to distinct populations of neurons located in specific hypothalamic regions, including the lateral hypothalamic area (LHA), a region implicated in feeding behaviour. Orexin A and orexin B bind to and activate two closely related G protein coupled receptors (GPCRs), termed Orexin 1 (OX1) and Orexin 2 (OX2) receptors. Dysfunction of the orexin peptide system has been linked to narcolepsy.
  • Cellular localizationRough endoplasmic reticulum. Cytoplasmic vesicle. Cell junction, synapse. Note: Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses.
  • Database links
  • Alternative names
    • HCRT antibody
    • HCRT1 antibody
    • HCRT2 antibody
    • hypocretin (orexin) neuropeptide precursor antibody
    • Hypocretin 1 antibody
    • Hypocretin 2 antibody
    • hypocretin antibody
    • NRCLP1 antibody
    • Orexin antibody
    • Orexin A antibody
    • Orexin B antibody
    • Orexin precursor antibody
    • OX antibody
    • PPORX antibody
    • PPOX antibody
    • PREPROOREXIN antibody
    see all

References for Anti-Orexin A antibody (ab35337)

This product has been referenced in:
  • Silva JP  et al. Regulation of adaptive behaviour during fasting by hypothalamic Foxa2. Nature 462:646-50 (2009). IHC-FoFr ; Mouse . Read more (PubMed: 19956259) »

See 1 Publication for this product

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Thank you for contacting us. Please choose antibodies from the following catalogue numbers: anti Ghrelin ab48285, ab117620, ab15861, ab57222, ab104307 These antibodies are tested in Indirect ELISA so are guaranteed for this application and ap...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"