Overview

  • Product name
  • Description
    Rabbit polyclonal to Orexin A
  • Specificity
    Immunohistochemical analysis of rat brain indicates a good specificity for this serum.
  • Tested applications
    Suitable for: IHC-FoFr, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to Cow Orexin A aa 14-33 conjugated to Keyhole Limpet Haemocyanin (KLH) (Glutaraldehyde).
    Sequence:

    CRLYELLHGAGNHAAGILTL


    Database link: P56717

Properties

Applications

Our Abpromise guarantee covers the use of ab6214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/1000. PubMed: 16052497

Following perfusion fixation with 4% PFA, cryoprotect the tissue in 20% sucrose overnight. Cut on cryostat sections for free floating IHC. Incubate primary antibody for 48 hrs at 4°C.

ELISA 1/1000.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    Orexin A (hypocretin 1) a 33 amino acid peptide and orexin B (hypocretin 2), a 28 amino acid peptide, are both derived from a common 130 amino acid precursor, prepro orexin. Orexin A and Orexin B stimulate food consumption when administered intracerebroventricularly to rats. Orexin gene expression in the brain is highly restricted to distinct populations of neurons located in specific hypothalamic regions, including the lateral hypothalamic area (LHA), a region implicated in feeding behaviour. Orexin A and orexin B bind to and activate two closely related G protein coupled receptors (GPCRs), termed Orexin 1 (OX1) and Orexin 2 (OX2) receptors. Dysfunction of the orexin peptide system has been linked to narcolepsy.
  • Cellular localization
    Rough endoplasmic reticulum. Cytoplasmic vesicle. Cell junction, synapse. Note: Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses.
  • Database links
  • Alternative names
    • HCRT antibody
    • HCRT1 antibody
    • HCRT2 antibody
    • hypocretin (orexin) neuropeptide precursor antibody
    • Hypocretin 1 antibody
    • Hypocretin 2 antibody
    • hypocretin antibody
    • NRCLP1 antibody
    • Orexin antibody
    • Orexin A antibody
    • Orexin B antibody
    • Orexin precursor antibody
    • OX antibody
    • PPORX antibody
    • PPOX antibody
    • PREPROOREXIN antibody
    see all

Anti-Orexin A antibody images

  • ab6214 staining Pan Orexin in mouse brain tissue sections by Immunohistochemistry (PFA fixed free floating sections). Tissue samples were fixed with paraformaldehyde, permeablized with 1% Triton X-100 and blocked with 1% BSA for 10 minutes at room temperature. The sample was incubated with primary antibody (1/2000 in 1X PBS + 0.1% Triton X-100 + 1% BSA) at 4°C for 24 hours. An Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

References for Anti-Orexin A antibody (ab6214)

This product has been referenced in:
  • Verma D  et al. Pancreatic polypeptide and its central Y4 receptors are essential for cued fear extinction and permanent suppression of fear. Br J Pharmacol 173:1925-38 (2016). IHC . Read more (PubMed: 26844810) »
  • Kerman IA  et al. Distinct populations of presympathetic-premotor neurons express orexin or melanin-concentrating hormone in the rat lateral hypothalamus. J Comp Neurol 505:586-601 (2007). Read more (PubMed: 17924541) »

See all 3 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (brain)
Antigen retrieval step
Heat mediated
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 15 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - Triton X-100 1%
Fixative
Paraformaldehyde
Username

Dr. Paloma Perez-Domper

Verified customer

Submitted Oct 27 2014

Thank you for your enquiry regarding the peptide immunogen used to generate ab6214 -- Rabbit polyclonal to Orexin A. I am afraid that we do not have stocks of this peptide here at Abcam so I am unable to send you a free sample for your control expe...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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