Orexin A (bovine, human, mouse, rat) (ab120212)

Overview

  • Product nameOrexin A (bovine, human, mouse, rat)
  • Description
    Neuropeptide involved in feeding and sleep
  • Biological descriptionHypothalamic neuropeptide involved in the regulation of feeding, sleep and wakefulness.
  • Purity> 99%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120212 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Orexin A (bovine, human, mouse, rat) images

    • ab6211 staining tyrosine hydroxylase in PC-12 cells treated with orexin A (ab120212), by ICC/IF. Decrease in expression of tyrosine hydroxylase correlates with increased concentration of orexin A, as described in literature.
      The cells were incubated at 37°C for 1h in media containing different concentrations of ab120212 (orexin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6211 (1/1000 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for Orexin A (bovine, human, mouse, rat) (ab120212)

    This product has been referenced in:

    See 0 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab120212.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"