• Product nameAnti-Orexin Receptor 1 antibody
    See all Orexin Receptor 1 primary antibodies
  • Description
    Rabbit polyclonal to Orexin Receptor 1
  • SpecificityNo cross reactivity with other proteins
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to a sequence from the C-terminus of human Orexin Receptor 1, identical to the related mouse and rat sequence.



Our Abpromise guarantee covers the use of ab68718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 48 kDa.Can be blocked with Orexin Receptor 1 peptide (ab188501).
IHC-P Use a concentration of 1 - 2 µg/ml.
IP Use a concentration of 5 µg/ml.


Anti-Orexin Receptor 1 antibody images

  • Anti-Orexin Receptor 1 antibody (ab68718) at 2 µg/ml + rat brain tissue lysate

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • ab68718 staining Orexin Receptor 1 in rat brain tissue by Immunohistochemistry (formalin fixed, paraffin embedded tissue section). Primary antibody used at 2µg/ml.
  • ICC/IF image of ab68718 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68718, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Orexin Receptor 1 was immunoprecipitated using 0.5mg Rat Brain tissue lysate, 5µg of Rabbit polyclonal to Orexin Receptor 1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Rat Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab68718.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 52kDa; Orexin Receptor 1

References for Anti-Orexin Receptor 1 antibody (ab68718)

This product has been referenced in:
  • Joshi D & Singh SK Localization and expression of Orexin A and its receptor in mouse testis during different stages of postnatal development. Gen Comp Endocrinol N/A:N/A (2016). WB, IHC ; Mouse . Read more (PubMed: 27174745) »
  • Chang X  et al. Orexin-A stimulates 3ß-hydroxysteroid dehydrogenase expression and cortisol production in H295R human adrenocortical cells through the AKT pathway. Int J Mol Med 34:1523-8 (2014). Human . Read more (PubMed: 25319929) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (Hypothalamus)
Gel Running Conditions Reduced Denaturing (Bis-Tris 4-12%)
Loading amount 20 µg
Specification Hypothalamus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

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Verified customer

Submitted Jul 01 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM Sodium citrate, 0.05% Tween-20, pH 6.0
Sample Rat Tissue sections (brain)
Specification brain
Permeabilization Yes - 0.1% triton-x, PBS
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 08 2014

Thank you for your enquiry.

IHC-FrFl is a very specific immunostaining application and most of our primary antibodies are not tested for thisi assay on a regular basis. However, it may well be that this antibody still works in IHC-FrFl but so...

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