Overview

  • Product nameAnti-Orexin Receptor 2 antibody
    See all Orexin Receptor 2 primary antibodies
  • Description
    Mouse polyclonal to Orexin Receptor 2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Pig
  • Immunogen

    Vector coding for a partial recombinant fusion protein, corresponding to amino acids 263/282 of Mouse Orexin Receptor 2. Target sequence used to make the antibody:

    VQRKWKQQQPVSQPRGSGQQS

    .

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab43445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 51 kDa. This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • FunctionNonselective, high-affinity receptor for both orexin-A and orexin-B neuropeptides (PubMed:9491897, PubMed:26950369). Triggers an increase in cytoplasmic Ca(2+) levels in response to orexin-A binding (PubMed:9491897, PubMed:26950369).
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • DomainThe N-terminal region is required for orexin signaling.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Hcr tr2 antibody
    • Hcrt receptor 2 antibody
    • Hcrtr2 antibody
    • Hypocretin (orexin) receptor 2 antibody
    • Hypocretin receptor 2 antibody
    • Hypocretin receptor type 2 antibody
    • Orexin 2 receptor antibody
    • Orexin A/Orexin B receptor antibody
    • Orexin receptor 2 antibody
    • Orexin receptor type 2 antibody
    • Orexin type 2 receptor antibody
    • Ox-2-R antibody
    • Ox2-R antibody
    • Ox2r antibody
    • OX2R_HUMAN antibody
    • Type 2 hypocretin receptor antibody
    • Type 2 orexin receptor antibody
    see all

Anti-Orexin Receptor 2 antibody images

  • All lanes : Anti-Orexin Receptor 2 antibody (ab43445) at 1/1000 dilution

    Lane 1 : a total protein extract from E coli with 50ng to 100 ng of a His tagged fusion protein of an irrelevant antigen
    Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (His tagged fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilution

    Predicted band size : 51 kDa
    Observed band size : 32.5 kDa (why is the actual band size different from the predicted?)

References for Anti-Orexin Receptor 2 antibody (ab43445)

This product has been referenced in:
  • Heydendael W  et al. Orexins/hypocretins act in the posterior paraventricular thalamic nucleus during repeated stress to regulate facilitation to novel stress. Endocrinology 152:4738-52 (2011). WB ; Rat . Read more (PubMed: 21971160) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"