Anti-Ornithine Carbamoyltransferase antibody (ab116265)

Overview

  • Product name
    Anti-Ornithine Carbamoyltransferase antibody
    See all Ornithine Carbamoyltransferase primary antibodies
  • Description
    Rabbit polyclonal to Ornithine Carbamoyltransferase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig, Saccharomyces cerevisiae, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 284-333 (YQVTMKTAKV AASDWTFLHC LPRKPEEVDD EVFYSPRSLV FPEAENRKWT) of Mouse Ornithine Carbamoyltransferase (NP_032795).

  • Positive control
    • Mouse liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab116265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-Ornithine Carbamoyltransferase antibody (ab116265) at 1 µg/ml + Mouse liver lysate at 10 µg

    Predicted band size: 39 kDa



    Gel concentration: 12%

References

ab116265 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Rat Cell lysate - other (ST14a cells lysate, mitochondrial fraction of ST14)
Gel Running Conditions
Reduced Denaturing (4-12% SDS-PAGE, Laemmli buffer with 10% β-Mercaptoethanol, 10 min at +55C denaturation. Immobilon-FL transfer membranes (Millipore), overnight transfer at +4C.)
Loading amount
15 µg
Specification
ST14a cells lysate, mitochondrial fraction of ST14
Blocking step
SEA Block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
Username

Svitlana Yablonska

Verified customer

Submitted May 24 2018

Thank you for contacting us.
I am sorry but I have as yet not recieved an update as to the restest of the antibody using a different sample (not HepG2). It may be that the tests have not been performed just yet but I will get in contact with the l...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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