Anti-Ornithine Carbamoyltransferase/OTC antibody (ab228646)
Key features and details
- Rabbit polyclonal to Ornithine Carbamoyltransferase/OTC
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Ornithine Carbamoyltransferase/OTC antibody
See all Ornithine Carbamoyltransferase/OTC primary antibodies -
Description
Rabbit polyclonal to Ornithine Carbamoyltransferase/OTC -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig -
Immunogen
Recombinant fragment within Human Ornithine Carbamoyltransferase/OTC (internal sequence). The exact sequence is proprietary.
Database link: P00480 -
Positive control
- IHC-P: Human colon carcinoma tissue. WB: A431 whole cell lysate, mouse liver tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/30000. Predicted molecular weight: 40 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/30000. Predicted molecular weight: 40 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Tissue specificity
Mainly expressed in liver and intestinal mucosa. -
Pathway
Nitrogen metabolism; urea cycle; L-citrulline from L-ornithine and carbamoyl phosphate: step 1/1. -
Involvement in disease
Ornithine carbamoyltransferase deficiency -
Sequence similarities
Belongs to the ATCase/OTCase family. -
Post-translational
modificationsAcetylation at Lys-88 negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 280887 Cow
- Entrez Gene: 5009 Human
- Entrez Gene: 18416 Mouse
- Entrez Gene: 397438 Pig
- Entrez Gene: 25611 Rat
- Omim: 300461 Human
- SwissProt: Q9N1U7 Cow
- SwissProt: P00480 Human
see all -
Alternative names
- EC 2.1.3.3 antibody
- MGC129967 antibody
- MGC129968 antibody
see all
Images
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Paraffin-embedded human colon carcinoma tissue stained for Ornithine Carbamoyltransferase/OTC with ab228646 at 1/250 dilution in immunohistochemical analysis.
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Anti-Ornithine Carbamoyltransferase/OTC antibody (ab228646) at 1/30000 dilution + Mouse liver tissue lysate at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 40 kDa10% SDS-PAGE
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Anti-Ornithine Carbamoyltransferase/OTC antibody (ab228646) at 1/500 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 40 kDa12% SDS-PAGE
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228646 has not yet been referenced specifically in any publications.