Anti-Ornithine Carbamoyltransferase antibody (ab228646)

Overview

  • Product name
    Anti-Ornithine Carbamoyltransferase antibody
    See all Ornithine Carbamoyltransferase primary antibodies
  • Description
    Rabbit polyclonal to Ornithine Carbamoyltransferase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Recombinant fragment within Human Ornithine Carbamoyltransferase (internal sequence). The exact sequence is proprietary.
    Database link: P00480

  • Positive control
    • IHC-P: Human colon carcinoma tissue. WB: A431 whole cell lysate, mouse liver tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab228646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/30000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/1000.

Target

Images

  • Paraffin-embedded human colon carcinoma tissue stained for Ornithine Carbamoyltransferase with ab228646 at 1/250 dilution in immunohistochemical analysis.

  • Anti-Ornithine Carbamoyltransferase antibody (ab228646) at 1/30000 dilution + Mouse liver tissue lysate at 20 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 40 kDa



    10% SDS-PAGE

  • Anti-Ornithine Carbamoyltransferase antibody (ab228646) at 1/500 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 40 kDa



    12% SDS-PAGE

References

ab228646 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - other (Brain homogenate, mitochondria from brain.)
Gel Running Conditions
Reduced Denaturing (4-12% SDS-PAGE, Laemmli buffer with 10% β-Mercaptoethanol,10 min at 55C denaturation.)
Loading amount
15 µg
Specification
Brain homogenate, mitochondria from brain.
Blocking step
SEA Block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
Username

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Verified customer

Submitted May 16 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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