Anti-Ornithine Carbamoyltransferase antibody (ab91418)

Overview

  • Product name
    Anti-Ornithine Carbamoyltransferase antibody
    See all Ornithine Carbamoyltransferase primary antibodies
  • Description
    Rabbit polyclonal to Ornithine Carbamoyltransferase
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Ornithine Carbamoyltransferase aa 71-98 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 5009

  • Positive control
    • HepG2 cell line lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab91418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000. Predicted molecular weight: 40 kDa.

Target

  • Tissue specificity
    Mainly expressed in liver and intestinal mucosa.
  • Pathway
    Nitrogen metabolism; urea cycle; L-citrulline from L-ornithine and carbamoyl phosphate: step 1/1.
  • Involvement in disease
    Ornithine carbamoyltransferase deficiency
  • Sequence similarities
    Belongs to the ATCase/OTCase family.
  • Post-translational
    modifications
    Acetylation at Lys-88 negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.1.3.3 antibody
    • MGC129967 antibody
    • MGC129968 antibody
    • MGC138856 antibody
    • OCTD antibody
    • Ornithine carbamoyltransferase mitochondrial antibody
    • Ornithine carbamoyltransferase, mitochondrial antibody
    • Ornithine transcarbamylase antibody
    • Otc antibody
    • OTC_HUMAN antibody
    • OTCase antibody
    see all

Images

  • Anti-Ornithine Carbamoyltransferase antibody (ab91418) at 1/100 dilution + HepG2 cell line lysate at 35 µg

    Predicted band size : 40 kDa
  • All lanes : Anti-Ornithine Carbamoyltransferase antibody (ab91418)

    Lane 1 : SW620 cell lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Rat liver tissue lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 40 kDa
  • Immunohistochemical staining of formalin-fixed and paraffin-embedded human hepatoarcinoma with ab91418, followed by HRP-conjugated secondary antibody and DAB staining.

  • Flow cytometric analysis of ATDC5 cells using ab91418 (green histogram) compared to a negative control cell (blue histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab91418 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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