Anti-Ornithine Decarboxylase antibody (ab66067)


  • Product nameAnti-Ornithine Decarboxylase antibody
    See all Ornithine Decarboxylase primary antibodies
  • Description
    Mouse monoclonal to Ornithine Decarboxylase
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human) with tag.

  • Positive control
    • Ornithine Decarboxylase transfected 293T cell lysate. IF/ICC: HepG2 cell line.



Our Abpromise guarantee covers the use of ab66067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration. The detection limit for recombinant tagged Ornithine Decarboxylase is approximately 30ng/ml as a capture antibody.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IHC-P 1/1000.


  • PathwayAmine and polyamine biosynthesis; putrescine biosynthesis via L-ornithine pathway; putrescine from L-ornithine: step 1/1.
  • Sequence similaritiesBelongs to the Orn/Lys/Arg decarboxylase class-II family.
  • Post-translational
    S-Nitrosylation inhibits the enzyme. S-Nitrosylated in vitro on 4 cysteine residues.
  • Information by UniProt
  • Database links
  • Alternative names
    • DCOR_HUMAN antibody
    • Dodc1 antibody
    • Odc 1 antibody
    • ODC antibody
    • Odc1 antibody
    • ODC2 antibody
    • Ornithine decarboxylase 1 antibody
    • Ornithine decarboxylase 2 antibody
    • Ornithine decarboxylase antibody
    • Ornithine decarboxylase structural 1 antibody
    • RNODC antibody
    see all

Anti-Ornithine Decarboxylase antibody images

  • ICC/IF image of ab66067 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66067, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Ornithine Decarboxylase antibody (ab66067) at 1 µg/ml

    Lane 1 : Ornithine Decarboxylase transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate.

    Lysates/proteins at 50 µg per lane.

    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
  • IHC-P image of Ornithine Decarboxylase (ab66067) on Colon cancer sections. The sections were fixed in Formaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 0.3% H2O2 solution for 10 mins at 22°C.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab66067 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab66067, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Hela cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Ornithine Decarboxylase antibody (ab66067)

This product has been referenced in:
  • Abu-Lubad M  et al. Chlamydia trachomatis inhibits inducible NO synthase in human mesenchymal stem cells by stimulating polyamine synthesis. J Immunol 193:2941-51 (2014). Human . Read more (PubMed: 25114102) »
  • Chen L  et al. Recoding RNA editing of AZIN1 predisposes to hepatocellular carcinoma. Nat Med 19:209-16 (2013). Read more (PubMed: 23291631) »

See all 3 Publications for this product

Product Wall

Thank you for the quick reply. The antibody is supplied at a concentration of 1 mg/ml so you would receive 100 ul. Please let me know if you have any further questions.

Thank you for contacting Abcam regarding ab66067.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon cancer)
Specification Colon cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Novacastra pH 6
Permeabilization No
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: 22°C

Dr. Aamir Ahmed

Verified customer

Submitted Oct 12 2010