Anti-ORP1 antibody (ab10151)

Overview

  • Product nameAnti-ORP1 antibody
    See all ORP1 primary antibodies
  • Description
    Goat polyclonal to ORP1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide: YWDRNYFNLPDIY, corresponding to C terminal amino acids 938-950 of Human ORP1.

  • Positive control
    • Human muscle lysate.
  • General notes


    Oxysterols are oxygenated derivatives of cholesterol that have a number of biological effects and play a key role in the maintenance of the body cholesterol balance. ORP1 is a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors that are believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notesOxysterols are oxygenated derivatives of cholesterol that have a number of biological effects and play a key role in the maintenance of the body cholesterol balance. ORP1 is a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors that are believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab10151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human ORP1 peptide (ab23188).

Target

References for Anti-ORP1 antibody (ab10151)

ab10151 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (Mouse and rat brain lysate, as well as cell lysate)
Loading amount 100 µg
Specification Mouse and rat brain lysate, as well as cell lysate
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted May 01 2008

I'm sorry to hear you are having a problem with ab10151. Although I do not have the information for the detailed protocol that you followed I would like to suggest the following modifications that may improve your result: I would block in 5% milk in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"