Anti-ORP150 antibody (ab56680)

Overview

  • Product nameAnti-ORP150 antibody
    See all ORP150 primary antibodies
  • Description
    Mouse monoclonal to ORP150
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: EVQYLLNKAK FTKPRPRPKD KNGTRAEPPL NASASDQGEK VIPPAGQTED AEPISEPEKV ETGSEPGDTE PLELGGPGAE PEQKEQSTGQ KRPLKNDEL, corresponding to amino acids 901-1000 of Human ORP150

Properties

Applications

Our Abpromise guarantee covers the use of ab56680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 111 kDa.
IHC-P Use a concentration of 1 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionHas a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding.
  • Tissue specificityHighly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. Highly expressed in liver and pancreas and lower expression in brain and kidney. Also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers.
  • Sequence similaritiesBelongs to the heat shock protein 70 family.
  • Cellular localizationEndoplasmic reticulum lumen.
  • Information by UniProt
  • Database links
  • Alternative names
    • 150 kDa oxygen regulated protein antibody
    • 150 kDa oxygen-regulated protein antibody
    • 170 kDa glucose regulated protein antibody
    • 170 kDa glucose-regulated protein antibody
    • DKFZp686N08236 antibody
    • FLJ94899 antibody
    • FLJ97572 antibody
    • glucose regulated protein 170 antibody
    • Grp 170 antibody
    • GRP-170 antibody
    • Grp170 antibody
    • HSP 12A antibody
    • HSP12A antibody
    • Hyou1 antibody
    • HYOU1_HUMAN antibody
    • Hypoxia up regulated 1 antibody
    • hypoxia up-regulated 1 precursor antibody
    • Hypoxia up-regulated protein 1 antibody
    • Hypoxia upregulated 1 antibody
    • Orp 150 antibody
    • ORP-150 antibody
    • Orp150 antibody
    • oxygen regulated protein (150kD) antibody
    see all

Anti-ORP150 antibody images

  • ORP150 antibody (ab56680) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human salivary gland.


  • Predicted band size : 111 kDa
    ORP150 antibody (ab56680) at 1ug/lane + MCF-7 cell lysate at 25ug/lane.
  • Overlay histogram showing HepG2 cells stained with ab56680 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56680, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-ORP150 antibody (ab56680)

ab56680 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Recombinant protein (Creative Biomart HOUY1-3255H)
Loading amount 1 µg
Specification Creative Biomart HOUY1-3255H
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Aug 22 2012

Thank you for submitting an Abreview of ab56680.
Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this anti...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"