Overview

  • Product nameAnti-ORP150 antibody
    See all ORP150 primary antibodies
  • Description
    Rabbit polyclonal to ORP150
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide selected from the center region of Human ORP150 conjugated to KLH (NP_006380).

  • Positive control
    • 293 cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab85233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 111 kDa (predicted molecular weight: 111 kDa).
ELISA 1/1000.

Target

  • FunctionHas a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding.
  • Tissue specificityHighly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. Highly expressed in liver and pancreas and lower expression in brain and kidney. Also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers.
  • Sequence similaritiesBelongs to the heat shock protein 70 family.
  • Cellular localizationEndoplasmic reticulum lumen.
  • Information by UniProt
  • Database links
  • Alternative names
    • 150 kDa oxygen regulated protein antibody
    • 150 kDa oxygen-regulated protein antibody
    • 170 kDa glucose regulated protein antibody
    • 170 kDa glucose-regulated protein antibody
    • DKFZp686N08236 antibody
    • FLJ94899 antibody
    • FLJ97572 antibody
    • glucose regulated protein 170 antibody
    • Grp 170 antibody
    • GRP-170 antibody
    • Grp170 antibody
    • HSP 12A antibody
    • HSP12A antibody
    • Hyou1 antibody
    • HYOU1_HUMAN antibody
    • Hypoxia up regulated 1 antibody
    • hypoxia up-regulated 1 precursor antibody
    • Hypoxia up-regulated protein 1 antibody
    • Hypoxia upregulated 1 antibody
    • Orp 150 antibody
    • ORP-150 antibody
    • Orp150 antibody
    • oxygen regulated protein (150kD) antibody
    see all

Anti-ORP150 antibody images

  • Anti-ORP150 antibody (ab85233) at 1/50 dilution + 293 cell line lysate at 35 µg

    Predicted band size : 111 kDa
    Observed band size : 111 kDa

References for Anti-ORP150 antibody (ab85233)

This product has been referenced in:
  • Wang Y  et al. Involvement of Oxygen-regulated Protein 150 in AMP-activated Protein Kinase-mediated Alleviation of Lipid-induced Endoplasmic Reticulum Stress. J Biol Chem 286:11119-31 (2011). WB ; Human . Read more (PubMed: 21296878) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Recombinant protein (Creative Biomart cat. nr. HYOU1-3255H)
Loading amount 1 µg
Specification Creative Biomart cat. nr. HYOU1-3255H
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 21 2012

Thank you for submitting an Abreview of ab85233.
Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this anti...

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Tut mir leid, dass die letzte email verloren gegangen ist. Leider scheint das manchmal zu passieren - gerade bei Instituten, die in ihren email-Adressen kein 'Uni-...'-Kürzel haben. Sollte das wieder passieren, fragen Sie einfach bei uns nach! Sie könn...

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Vielen Dank für Ihre Anfrage. Bitte entschuldigen Sie, dass meine Antwort so lange auf sich warten ließ. Ich habe mit dem Labor, in dem der Antikörper getestet wurde, Rücksprache wegen der 90 kDa-Bande gehalten. Wir wissen leider nicht, worum es sic...

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Vielen Dank für Ihre Antwort und für diese weiteren Informationen.  Bitte entschuldigen Sie die Verzögerung. Ich habe nun eine kostenfreie Lieferung des Blockpeptides für ORP150 arrangiert. Sie hat die Referenznummer xxxxx und sollte in den nächste...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"