Overview

  • Product nameAnti-ORP8 antibody
    See all ORP8 primary antibodies
  • Description
    Rabbit polyclonal to ORP8
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 755-804 (

    YSSPEPDIQDSSGSEAQSVKPSTRRKKGIELGDIQSSIESIKQTQEEIKR

    ) of Human ORP8 (NP_001003712).

  • Positive control
    • OVCAR3 cell lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal prostate

Properties

Applications

Our Abpromise guarantee covers the use of ab99069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 101 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceORP8 is a member of the oxysterol-binding protein related protein family. In humans, the gene family consists of 12 members, and extensive splice variation increases the number of encoded protein products substantially. The ORPs have been implicated as sterol sensors that regulate a number of cellular functions including sterol and neutral lipid metabolism, intracellular lipid transport, membrane trafficking and cell signaling. ORP8 has been recently shown to act as a sterol sensor that affects the reverse cholesterol transport process via modulation of ABCA1 expression and macrophage cholesterol efflux.
  • Cellular localizationEndoplasmic reticulum
  • Database links
  • Alternative names
    • DKFZp686A11164 antibody
    • KIAA1451 antibody
    • MGC126578 antibody
    • MGC133203 antibody
    • MST120 antibody
    • MSTP120 antibody
    • ORP 8 antibody
    • OSBP related protein 8 antibody
    • OSBP10 antibody
    • OSBPL8 antibody
    • Oxysterol binding protein like 8 antibody
    • Oxysterol binding protein like protein 8 antibody
    • Oxysterol binding protein related protein 8 antibody
    see all

Anti-ORP8 antibody images

  • IHC image of ORP8 staining in Human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab99069, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-ORP8 antibody (ab99069) at 1 µg/ml + OVCAR3 cell lysate at 10 µg

    Predicted band size : 101 kDa
    Gel concentration: 6-18%

References for Anti-ORP8 antibody (ab99069)

ab99069 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"