Overview

  • Product nameAnti-OS9 antibody
    See all OS9 primary antibodies
  • Description
    Rabbit polyclonal to OS9
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human OS9.

    (Peptide available as ab23317.)

  • Positive control
    • WB: HeLa, HEK293 and NIH3T3 whole cell lysates and mouse heart, spleen, and liver tissue lysates. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab19853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
IP Use at an assay dependent dilution.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionLectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.
  • Tissue specificityUbiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma OSA-CL and rhabdomyosarcoma RH30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.
  • Sequence similaritiesBelongs to the OS-9 family.
    Contains 1 PRKCSH domain.
  • Post-translational
    modifications
    Intramolecular disulfide bonds.
    Isoform 1 and isoform 2 are N-glycosylated.
  • Cellular localizationEndoplasmic reticulum lumen.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amplified in osteosarcoma 9 antibody
    • amplified in osteosarcoma antibody
    • ERLEC2 antibody
    • OS-9 antibody
    • Os9 antibody
    • OS9_HUMAN antibody
    • Osteosarcoma amplified 9 endoplasmic reticulum lectin antibody
    • Protein OS-9 antibody
    see all

Anti-OS9 antibody images

  • IHC image of OS9 staining in human hippocampus FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19853, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • All lanes : Anti-OS9 antibody (ab19853) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 4 : Spleen (Mouse) Tissue Lysate
    Lane 5 : Liver (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 76 kDa
    Observed band size : 76 kDa
    Additional bands at : 30 kDa,74 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes
  • ICC/IF image of ab19853 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19853, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-OS9 antibody (ab19853)

This product has been referenced in:
  • Iwamoto S  et al. Reconstructed glycan profile for evaluation of operating status of the endoplasmic reticulum glycoprotein quality control. Glycobiology 23:121-31 (2013). Read more (PubMed: 22975980) »
  • Williams JM  et al. The ERdj5-Sel1L complex facilitates cholera toxin retrotranslocation. Mol Biol Cell 24:785-95 (2013). Human . Read more (PubMed: 23363602) »

See all 5 Publications for this product

Product Wall

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Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeP3B)
Total protein in input 6e+006 cells
Specification HeP3B
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

Submitted Dec 22 2010

Abreviews
Application Western blot
Sample Dog Tissue lysate - other (pancreatic microsomes)
Loading amount 10 µg
Specification pancreatic microsomes
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Dr. Felicity Alcock

Verified customer

Submitted Feb 11 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Lung, Liver)
Loading amount 25 µg
Specification Lung, Liver
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 20 2009

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells transfected with OS9 tv2-HA)
Specification HeLa cells transfected with OS9 tv2-HA
Fixative Methanol
Username

Dr. Felicity Alcock

Verified customer

Submitted Feb 05 2008

Abreviews
Application Western blot
Sample Rat Tissue lysate - other (Rat liver membranes)
Loading amount 10 µg
Specification Rat liver membranes
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Dr. Felicity Alcock

Verified customer

Submitted Jan 28 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa cells transfected with OS9 tv2-HA)
Total protein in input 600000 cells
Specification HeLa cells transfected with OS9 tv2-HA
Treatment S35-labelled
Immuno-precipitation step Protein A
Username

Dr. Felicity Alcock

Verified customer

Submitted Jan 24 2008

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 100000 cells
Specification HeLa
Treatment lane 1 - OS9 siRNA lane 2 - laminA siRNA lane 3 - no treatment lane 4 - transfected with OS9 tv2
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Dr. Felicity Alcock

Verified customer

Submitted Jan 23 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HT1080 cells)
Loading amount 10 µg
Specification HT1080 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Ms. Xiaoli Shi

Verified customer

Submitted Nov 30 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"