1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/250.
Application notesIs unsuitable for Flow Cyt.
FunctionLectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.
Tissue specificityUbiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma OSA-CL and rhabdomyosarcoma RH30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.
Sequence similaritiesBelongs to the OS-9 family. Contains 1 PRKCSH domain.
Post-translational modificationsIntramolecular disulfide bonds. Isoform 1 and isoform 2 are N-glycosylated.
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using ab109510 at a dilution of 1/100.
References for Anti-OS9 antibody [EPR4272(2)] (ab109510)
This product has been referenced in:
Sun S et al. Sel1L is indispensable for mammalian endoplasmic reticulum-associated degradation, endoplasmic reticulum homeostasis, and survival. Proc Natl Acad Sci U S A111:E582-91 (2014).
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