Overview

  • Product name
  • Description
    Goat polyclonal to OSBP1
  • Specificity
    Ab31107 recognises OSBP1.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide:

    C-KHQLEETKKEKRTRIP

    , corresponding to Internal sequence amino acids 395-410 of Human OSBP1.

  • Positive control
    • Human placenta lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab31107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/32000.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 89 kDa).

Target

  • Function
    Binds cholesterol and a range of oxysterols. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylate ERK1/2, whereas 25-hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing ABCA1 stability.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the OSBP family.
    Contains 1 PH domain.
  • Domain
    The PH and Ala/Gly-rich domains control cholesterol binding without affecting 25-hydroxycholesterol binding.
    The second coiled-coil domain is required for interaction with the tyrosine phosphatase.
  • Cellular localization
    Cytoplasm. Golgi apparatus membrane. When bound to oxysterols, translocates to the periphery of Golgi membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • OSBP 1 antibody
    • OSBP antibody
    • OSBP1_HUMAN antibody
    • Oxysterol binding protein 1 antibody
    • Oxysterol-binding protein 1 antibody
    see all

Images

  • Anti-OSBP1 antibody (ab31107) at 0.1 µg/ml + Human Placenta lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 89 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

References

This product has been referenced in:
  • Auweter SD  et al. Quantitative mass spectrometry catalogues salmonella pathogenicity island-2 effectors and identifies their cognate host binding partners. J Biol Chem 286:24023-35 (2011). WB ; Human . Read more (PubMed: 21566117) »
  • Zerbinatti CV  et al. Oxysterol-binding protein-1 (OSBP1) modulates processing and trafficking of the amyloid precursor protein. Mol Neurodegener 3:5 (2008). WB ; Human . Read more (PubMed: 18348724) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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