The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 89 kDa).
FunctionBinds cholesterol and a range of oxysterols. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylate ERK1/2, whereas 25-hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing ABCA1 stability.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the OSBP family. Contains 1 PH domain.
DomainThe PH and Ala/Gly-rich domains control cholesterol binding without affecting 25-hydroxycholesterol binding. The second coiled-coil domain is required for interaction with the tyrosine phosphatase.
Cellular localizationCytoplasm. Golgi apparatus membrane. When bound to oxysterols, translocates to the periphery of Golgi membranes.