Overview

  • Product name
  • Description
    Rabbit polyclonal to OSGEPL1
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Protein Epitope Signature Tag, corresponding to amino acids 55 - 185 of Human OSGEPL1.

  • Positive control
    • Human kidney tissue
      RT-4 and U-251 MG lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Function
    Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine.
  • Sequence similarities
    Belongs to the KAE1 / YgjD family.
  • Information by UniProt
  • Database links
  • Alternative names
    • O sialoglycoprotein endopeptidase like 1 antibody
    • O sialoglycoprotein endopeptidase like protein 1 antibody
    • osgepl1 antibody
    • OSGP2_HUMAN antibody
    • Probable O sialoglycoprotein endopeptidase 2 antibody
    • Probable tRNA threonylcarbamoyladenosine biosynthesis protein OSGEPL1 antibody
    • Putative sialoglycoprotease type 2 antibody
    • Qri7 antibody
    • t(6)A37 threonylcarbamoyladenosine biosynthesis protein OSGEPL1 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in mitochondria. Recommended concentration of ab122125 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-OSGEPL1 antibody (ab122125) at 1/250 dilution

    Lane 1 : RT-4 lysate
    Lane 2 : U-251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate
  • ab122125, at a 1/15 dilution, staining OSGEPL1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

References

ab122125 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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