Overview

  • Product name
    Anti-ospA antibody
  • Description
    Rabbit polyclonal to ospA
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Other
  • Immunogen

    MBP-fusion protein corresponding to Borrelia burgdorferi ospA protein.

  • Positive control
    • Borrelia burgdorferi recombinant ospA protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab106081 is cross-adsorbed against MBP from monospecific antiserum by chromatography.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab106081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 29 kDa.

Target

  • Relevance
    Outer-Surface Protein A (OspA), a lipoprotein from Borrelia burgdorferi encoded on its Plasmid lp54, is a major component of the spirochete's extracellular matrix. OspA probably serves as a lipid-anchor. The spirochetes migrate from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. Upon transmission of the spirochete from the Ixodes tick to mammalian host, the transcript level of OspA can change. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host. B. burgdorferi can attach to (and also differentially express antigens in) diverse tissues within the vertebrate host and the tick vector, suggesting that physiological factors other than pH and temperature may play roles in modulating B. burgdorferi gene expression.
  • Cellular localization
    Cell outer membrane; Lipid-anchor.
  • Alternative names
    • OSPA antibody
    • outer surfce protein A antibody

Images

  • All lanes : Anti-ospA antibody (ab106081) at 1/1000 dilution

    Lane 1 : MBP-ospA fusion protein
    Lane 2 : MBP alone

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    Goat-anti-Rabbit HRP at 1/40000 dilution

    Predicted band size : 29 kDa

References

ab106081 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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