The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
RelevanceThe OSR1 (odd skipped related 1) gene encodes a zinc-finger transcription factor that is expressed in the germ ring mesendoderm and subsequently in the endoderm and intermediate mesoderm, prior to the expression of definitive kidney or blood/vascular markers. Osr1 expression marks a multi-potent population of intermediate mesoderm that undergoes progressive restriction to an Osr1-dependent nephron progenitor compartment within the mammalian kidney. Osr1 expression is regulated by Runx2 and Ikzf1, which are known as master-gene of osteogenesis and hematopoiesis, respectively.
All lanes : Anti-OSR1 antibody (ab74003) at 1 µg/ml
Lane 1 : Human colon tissue lysate - total protein (ab30051) Lane 2 : Colon (Mouse) Tissue Lysate Lane 3 : Colon (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 30 kDa Observed band size : 30 kDa Additional bands at : 60 kDa,70-75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSR1 antibody (ab74003)This image is courtesy of an abreview submitted by Aamir Ahmed, University College London, United Kingdom.
IHC-P image of OSR1 antibody (ab74003) on Prostate sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 0.3% H2O2 solution for 10 minutes at 22°C.