The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionEssential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for bile acid export from enterocytes into portal blood. Efficiently transports the major species of bile acids.
Tissue specificityWidely expressed with a high expression in ileum. Expressed in testis, colon, liver, small intestine, kidney, ovary and adrenal gland; and at low levels in heart, lung, brain, pituitary, thyroid gland, uterus, prostate, mammary gland and fat.
Sequence similaritiesBelongs to the OST-beta family.
Cellular localizationCell membrane. Mainly restricted to the lateral and basal membranes of ileal enterocytes.
ab121285, at a 1/25 dilution, staining OST-beta in glandular cells of paraffin-embedded Human colon tissue by immunohistochemistry.
Western blot - Anti-OST-beta antibody (ab121285)
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
References for Anti-OST-beta antibody (ab121285)
has not yet been referenced specifically in any publications.
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