Overview

  • Product name
    Anti-OST-beta antibody
  • Description
    Rabbit polyclonal to OST-beta
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: VLHLDEAKDH NSLNNLRETL LSEKPNLAQV ELELKERDVL SVFLPDVPET E, corresponding to amino acids 77-127 of Human OST-beta.

  • Positive control
    • Human colon tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Essential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for bile acid export from enterocytes into portal blood. Efficiently transports the major species of bile acids.
  • Tissue specificity
    Widely expressed with a high expression in ileum. Expressed in testis, colon, liver, small intestine, kidney, ovary and adrenal gland; and at low levels in heart, lung, brain, pituitary, thyroid gland, uterus, prostate, mammary gland and fat.
  • Sequence similarities
    Belongs to the OST-beta family.
  • Cellular localization
    Cell membrane. Mainly restricted to the lateral and basal membranes of ileal enterocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Organic solute transporter subunit beta antibody
    • OST-beta antibody
    • OSTB antibody
    • OSTB_HUMAN antibody

Images

  • ab121285, at a 1/25 dilution, staining OST-beta in glandular cells of paraffin-embedded Human colon tissue by immunohistochemistry.
  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

References

ab121285 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Huh-7 human hepatocyte-derived cell line)
Permeabilization
Yes - Triton X-100 0.1%
Specification
Huh-7 human hepatocyte-derived cell line
Blocking step
Serum as blocking agent for 17 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Formaldehyde
Username

Melina Malinen

Verified customer

Submitted Sep 15 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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