Overview

  • Product name
    Anti-OSTA antibody
  • Description
    Rabbit polyclonal to OSTA
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 305-335 of Human OSTA (Q86UW1)

  • Positive control
    • 293 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 38 kDa.

Target

  • Relevance
    OSTA is an essential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for bile acid export from enterocytes into portal blood. It efficiently transports the major species of bile acids.
  • Cellular localization
    Cell membrane; Multi-pass membrane protein
  • Database links
  • Alternative names
    • Organic solute transporter subunit alpha antibody
    • OST-alpha antibody

Images

  • Anti-OSTA antibody (ab103442) at 1/100 dilution + 293 cell lysate at 35 µg

    Predicted band size : 38 kDa

References

ab103442 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Cryopreserved human hepatocytes)
Permeabilization
Yes - Triton X-100 0.1%
Specification
Cryopreserved human hepatocytes
Blocking step
10% serum together with 0.4% BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde
Username

Melina Malinen

Verified customer

Submitted Oct 02 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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