Overview

  • Product nameAnti-Osteocalcin antibody
    See all Osteocalcin primary antibodies
  • Description
    Rabbit polyclonal to Osteocalcin
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Osteocalcin (internal sequence).
    Database link: NP_031567

Properties

Applications

Our Abpromise guarantee covers the use of ab93876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 11 kDa. PubMed: 24753072
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionConstitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • Sequence similaritiesBelongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • Post-translational
    modifications
    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BGLAP antibody
    • BGP antibody
    • Bone gamma carboxyglutamate (gla) protein antibody
    • Bone gamma carboxyglutamate gla protein osteocalcin antibody
    • Bone gamma carboxyglutamate protein antibody
    • Bone Gla protein antibody
    • Gamma carboxyglutamic acid containing protein antibody
    • Gamma-carboxyglutamic acid-containing protein antibody
    • OC antibody
    • OCN antibody
    • OSTCN_HUMAN antibody
    • Osteocalcin antibody
    • OTTHUMP00000016586 antibody
    • PMF1 antibody
    see all

Anti-Osteocalcin antibody images

  • IHC image of ab93876 staining in Mouse bone formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93876, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Osteocalcin antibody (ab93876)

This product has been referenced in:
  • Dang PN  et al. Controlled Dual Growth Factor Delivery From Microparticles Incorporated Within Human Bone Marrow-Derived Mesenchymal Stem Cell Aggregates for Enhanced Bone Tissue Engineering via Endochondral Ossification. Stem Cells Transl Med 5:206-17 (2016). Human . Read more (PubMed: 26702127) »
  • Qu X  et al. MiR-132-3p Regulates the Osteogenic Differentiation of Thoracic Ligamentum Flavum Cells by Inhibiting Multiple Osteogenesis-Related Genes. Int J Mol Sci 17:N/A (2016). WB ; Human . Read more (PubMed: 27556448) »

See all 10 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Mouse Calvarial Tissue)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 25 µg
Treatment With Insulin as Positive Control
Specification Mouse Calvarial Tissue
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Abcam user community

Verified customer

Submitted Mar 29 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Calvarial bone)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization No
Specification Calvarial bone
Blocking step BSA and horse serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Jakob Townsend

Verified customer

Submitted Dec 16 2015

DISCOUNT CODE: XXXXXXXX
Expiration date: 03-09-2012

I am very pleased to hear you would like to accept our offer and test ab93876 in ICC/IF. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the exp...

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According to the expression profile of these proteins:

Osteopontin is highly expressed in mouse bone tissue:

http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Mm.288474

...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"