Overview

  • Product name
  • Description
    Rabbit polyclonal to Osteocalcin
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Osteocalcin (internal sequence).
    Database link: NP_031567

Properties

Applications

Our Abpromise guarantee covers the use of ab93876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 11 kDa. PubMed: 24753072
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • Sequence similarities
    Belongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • Post-translational
    modifications
    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BGLAP antibody
    • BGP antibody
    • Bone gamma carboxyglutamate (gla) protein antibody
    • Bone gamma carboxyglutamate gla protein osteocalcin antibody
    • Bone gamma carboxyglutamate protein antibody
    • Bone Gla protein antibody
    • Gamma carboxyglutamic acid containing protein antibody
    • Gamma-carboxyglutamic acid-containing protein antibody
    • OC antibody
    • OCN antibody
    • OSTCN_HUMAN antibody
    • Osteocalcin antibody
    • OTTHUMP00000016586 antibody
    • PMF1 antibody
    see all

Anti-Osteocalcin antibody images

  • IHC image of ab93876 staining in Mouse bone formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93876, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Osteocalcin antibody (ab93876)

This product has been referenced in:
  • Zhang X  et al. Dickkopf-related protein 3 negatively regulates the osteogenic differentiation of rat dental follicle cells. Mol Med Rep 15:1673-1681 (2017). WB ; Rat . Read more (PubMed: 28259940) »
  • Gao SY  et al. Zoledronate suppressed angiogenesis and osteogenesis by inhibiting osteoclasts formation and secretion of PDGF-BB. PLoS One 12:e0179248 (2017). WB ; Mouse . Read more (PubMed: 28594896) »

See all 15 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue (ectopic bone in muscle)
Permeabilization
Yes - 0,1% triton, 5 minutes
Specification
ectopic bone in muscle
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Anna Giovenzana

Verified customer

Submitted May 30 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse Calvarial Tissue)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
25 µg
Treatment
With Insulin as Positive Control
Specification
Mouse Calvarial Tissue
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Abcam user community

Verified customer

Submitted Mar 29 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Calvarial bone)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Specification
Calvarial bone
Blocking step
BSA and horse serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Formaldehyde
Username

Jakob Townsend

Verified customer

Submitted Dec 16 2015

DISCOUNT CODE: XXXXXXXX
Expiration date: 03-09-2012

I am very pleased to hear you would like to accept our offer and test ab93876 in ICC/IF. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the exp...

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According to the expression profile of these proteins:

Osteopontin is highly expressed in mouse bone tissue:

http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Mm.288474

...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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