• Product nameAnti-Osteocalcin antibody [OC4-30]
    See all Osteocalcin primary antibodies
  • Description
    Mouse monoclonal [OC4-30] to Osteocalcin
  • Tested applicationsSuitable for: WB, IHC-Fr, Sandwich ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Sheep, Rabbit, Goat, Cow, Dog, Human, Pig
    Does not react with: Mouse
  • Immunogen

    Full length protein corresponding to Cow Osteocalcin.

  • EpitopeIncludes the gamma-carboxylated residue at position 17.



Our Abpromise guarantee covers the use of ab13418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 12.0 kDa.
IHC-Fr Use a concentration of 10 µg/ml.
Sandwich ELISA Use a concentration of 1 - 10 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 20537381
IHC-P Use a concentration of 10 µg/ml.


  • FunctionConstitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • Sequence similaritiesBelongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • Post-translational
    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BGLAP antibody
    • BGP antibody
    • Bone gamma carboxyglutamate (gla) protein antibody
    • Bone gamma carboxyglutamate gla protein osteocalcin antibody
    • Bone gamma carboxyglutamate protein antibody
    • Bone Gla protein antibody
    • Gamma carboxyglutamic acid containing protein antibody
    • Gamma-carboxyglutamic acid-containing protein antibody
    • OC antibody
    • OCN antibody
    • OSTCN_HUMAN antibody
    • Osteocalcin antibody
    • OTTHUMP00000016586 antibody
    • PMF1 antibody
    see all

Anti-Osteocalcin antibody [OC4-30] images

  • Immunofluorescence analysis of 1 month osteoblastic-differentiated Human dental pulp stem cells, staining Osteocalcin with ab13418 at 1/80 dilution. A FITC-conjugated anti-mouse IgG was used as the secondary antibody.
  • ab13418 staining Osteocalcin in Human bone marrow stem cells tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was blocked with 5%BSA + TBS  + 0,5%Tween for 10 minutes. Samples were incubated with primary antibody (1/80) for 14 hours. An undiluted Biotin-conjugated Donkey polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-Osteocalcin antibody [OC4-30] (ab13418)

This product has been referenced in:
  • Lu H  et al. Hepcidin promotes osteogenic differentiation through the bone morphogenetic protein 2/small mothers against decapentaplegic and mitogen-activated protein kinase/P38 signaling pathways in mesenchymal stem cells. Mol Med Rep 11:143-50 (2015). WB ; Rat . Read more (PubMed: 25351366) »
  • Paino F  et al. Histone deacetylase inhibition with valproic acid downregulates osteocalcin gene expression in human dental pulp stem cells and osteoblasts: evidence for HDAC2 involvement. Stem Cells 32:279-89 (2014). IF . Read more (PubMed: 24105979) »

See all 16 Publications for this product

Product Wall

Unfortunately, none of the antibodies requested have been tested in sandwich ELISA. They are validated for either direct or indirect ELISA, but they have not been paired to another antibody to work together in the application.


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Gracias por contactarnos.

Tenemos en el catálogo, como bien indicas, tres anticuerpos contra Osteocalcina testados en humano mediante inmunocitoquímica e immunohistoquímica: ab13418, ab13420 y ab13421.

Todos nue...

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Thank you for contacting us.

Please buy ab7097.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.

Once the incubation with secondary antibody is finished then you need to add tertiary antibody which is in your case would be anti rat IgG-HRP. Please incubate for 1 hour at room temperature. Please make sure the t...

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Thank you for contacting us.

We have a Trypsin Enzymatic Antigen Retrieval Solution in our catalogue with reference ab970 (http://www.abcam.com/ab970).

I hope this information is helpful to you. Please do not hesitate to contact u...

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The protocol seems fine to me. However the following updates can be tried

- 0.3% H2O2 can be incubated for 10-15 minutes only.
- The endogenous peroxidases can be blocked after addition of primary antibody. Please check whether the tissue ...

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Thank you for contacting us.

I have asked few questions in my previous email so please send be answers asap. It would be very helpful if you could send me your step by step protocol as I suspect the protocol might needs updates.


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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;


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This is correct.
Please note that some antibodies work in both IHC-P and IHC-GMA, some do work in IHC-P or IHC-GMA only.
I hope this helps.

Yes we have a recommended protocol for resin (GMA) embedding.
The link to the protocol is: http://www.abcam.com/index.html?pageconfig=resource&rid=11464.
Note that you don't need the antigen retrieval step in your IHC protocol when working ...

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