Overview

  • Product nameAnti-Osteocalcin antibody [OCG3]
    See all Osteocalcin primary antibodies
  • Description
    Mouse monoclonal [OCG3] to Osteocalcin
  • Tested applicationsSuitable for: WB, IHC-Fr, IHC-P, Sandwich ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Sheep, Rabbit, Goat, Chicken, Cow, Dog, Human, Pig
    Does not react with: Mouse
  • Immunogen

    Full length protein corresponding to Cow Osteocalcin.
    Database link: P02820

  • EpitopeResidues 21-31
  • Positive control
    • Normal bovine bone tissue
  • General notes

    Though we have found ab13420 does not react with mouse sample in our lab, there is a report from a reviewer that got positive result with mouse sample in IHC-Fr. However, this information needs to be confirmed further.

     

    Although this antibody does work in IHC-P, we have found that ab13418 typically gives a more robust signal. Therefore, if you plan on using this antibody in IHC-P, we recommend ab13418 as an alternative option.

Properties

Applications

Our Abpromise guarantee covers the use of ab13420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 12 kDa.

Use under reducing or non-reducing conditions.

IHC-Fr Use a concentration of 1 - 10 µg/ml.
IHC-P Use a concentration of 10 µg/ml. Enzymatic retrieval with Proteinase K is recommended.
Sandwich ELISA Use a concentration of 1 - 10 µg/ml.

For use in sandwich ELISA with ab13421

ICC/IF Use at an assay dependent concentration. PubMed: 21396708

Target

  • FunctionConstitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • Sequence similaritiesBelongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • Post-translational
    modifications
    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BGLAP antibody
    • BGP antibody
    • Bone gamma carboxyglutamate (gla) protein antibody
    • Bone gamma carboxyglutamate gla protein osteocalcin antibody
    • Bone gamma carboxyglutamate protein antibody
    • Bone Gla protein antibody
    • Gamma carboxyglutamic acid containing protein antibody
    • Gamma-carboxyglutamic acid-containing protein antibody
    • OC antibody
    • OCN antibody
    • OSTCN_HUMAN antibody
    • Osteocalcin antibody
    • OTTHUMP00000016586 antibody
    • PMF1 antibody
    see all

Anti-Osteocalcin antibody [OCG3] images

  • Detection of human osteocalcin by ab13420
  • Image from Strauß S et al., PloS One. 2012;7(12):14. Fig 8.; doi: 10.1371/journal.pone.0051264

References for Anti-Osteocalcin antibody [OCG3] (ab13420)

This product has been referenced in:
  • Wehrle-Martinez AS  et al. Osteocalcin and Osteonectin Expression in Canine Osteosarcoma. Vet Pathol 53:781-7 (2016). Read more (PubMed: 26926085) »
  • Lambert LJ  et al. Increased trabecular bone and improved biomechanics in an osteocalcin-null rat model created by CRISPR/Cas9 technology. Dis Model Mech 9:1169-1179 (2016). WB, IHC-P ; Rat . Read more (PubMed: 27483347) »

See all 18 Publications for this product

Product Wall

Application Immunocytochemistry
Sample Human Cultured Cells (PULP)
Permeabilization No
Specification PULP
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Ms. kyungjoo seong

Verified customer

Submitted Nov 30 2016

Gracias por contactarnos.

Tenemos en el catálogo, como bien indicas, tres anticuerpos contra Osteocalcina testados en humano mediante inmunocitoquímica e immunohistoquímica: ab13418, ab13420 y ab13421.

Todos nue...

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Thank you very much for contacting us with your enquiry.

The AMELX protein ab139212 can be diluted in any buffer. However, all of our products are intended for research use only and are not recommended for in vitro studies. This protein conta...

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Vielen Dank für Ihre Anfrage.

Die Lieferung über das Wochenende erfolgt bei uns regelmaessig, ist also kein Grund zur Beunruhigung, da wir erstens den Antikörper mit Kuhlakkus versenden, zweitens FedEx die Produkte im Kühlh...

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I am sorry these products did not perform as stated on the datasheet and for the inconvenience th...

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I am sorry to hear about the shortage and sincerely apologize about that.


Your free of charge replacement for ab13420 will be shipped with your next order. The free replacement is shipping at full...

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I had sent a reply to this on Wednesday, but it seems that it did not get to your inbox. Could you please let me know once you receive this message? My previous message is below:

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Thank you very much for contacting us with your question.

The osteocalcin antibody ab13420 is provided at a concentration of 2 mg/mL, and we recommend using the antibody at 10 ug/mL in IHC-P. So, the antibody will need to be diluted 1:200 bef...

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DISCOUNT CODE: XXXXXXXXXX
Expiration date: 21-09-2012

I am very pleased to hear you would like to accept our offer and test ab13420 in [GUNIEA PIG]. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before...

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Nous somme vraiment désolés de ne pas avoir pu vous fournir un bon anticorps anti-osteocalcine. J'ai demandé à notre service comptabilité de mettre en place un avoir référence CN.

Votre référence CN est ******.

Voici les mani...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"