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Full length protein corresponding to Cow Osteocalcin.
Database link: P02820
Though we have found ab13420 does not react with mouse sample in our lab, there is a report from a reviewer that got positive result with mouse sample in IHC-Fr. However, this information needs to be confirmed further.
Although this antibody does work in IHC-P, we have found that ab13418 typically gives a more robust signal. Therefore, if you plan on using this antibody in IHC-P, we recommend ab13418 as an alternative option.
Our Abpromise guarantee covers the use of ab13420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 12 kDa.
Use under reducing or non-reducing conditions.
|IHC-Fr||Use a concentration of 1 - 10 µg/ml.|
|IHC-P||Use a concentration of 10 µg/ml. Enzymatic retrieval with Proteinase K is recommended.|
|Sandwich ELISA||Use a concentration of 1 - 10 µg/ml.
For use in sandwich ELISA with ab13421
|ICC/IF||Use at an assay dependent concentration. PubMed: 21396708|
Image from Strauß S et al., PloS One. 2012;7(12):14. Fig 8.; doi: 10.1371/journal.pone.0051264